Antibodies

Question 1

substances, specifically glycoproteins, produced by B lymphocytes or plasma cells (matured or differentiated B lymphocytes) in response to antigenic stimulation

Answer 1

ANTIBODIES

Question 2

other term for ANTIBODIES

Answer 2

Immunoglobulins

Question 3

Previously known as _____ ________ since it was
found to be concentrated on gamma region of serum protein electrophoresis.

Answer 3

gamma globulins

Question 4

is the same as the antibody classes. Antibody classes depends on their heavy chain present and the heavy chain of the antibodies is basically used to name the antibodies

Answer 4

Isotype

Question 5

5 HEAVY CHAINS

Answer 5

✓ Gamma (IgG)
✓ Mu (IgM)
✓ Alpha (IgA)
✓ Delta (IgD)
✓ Epsilon (IgE)

Question 6

Gamma

Answer 6

IgG

Question 7

Mu

Answer 7

IgM

Question 8

Alpha

Answer 8

IgA

Question 9

Delta

Answer 9

IgD

Question 10

Epsilon

Answer 10

IgE

Question 11

determined by the difference in the antibody structure in the constant region, specifically the amino acid sequence

Answer 11

Allotype

Question 12

determined according to the difference in the variable region of the antibody

Answer 12

Idiotype

Question 13

Composed of 2 identical light chains and 2 identical heavy chains (different chains are not allowed)

Answer 13

Four (4) polypeptide chains

Question 14

Example of Light chains

Answer 14

Kappa & Lambda

Question 15

synthesized with regulation of gene located in
chromosome 2

Answer 15

Kappa

Question 16

formation of this is regulated by the gene located in chromosome 22

Answer 16

Lambda

Question 17

synthesis of Mu, alpha, delta, epsilon regulated by the chromosome 14

Answer 17

Heavy chains

Question 18

Both light and heavy chains are held together
by

Answer 18

COVALENT DISULFIDE BONDS

Question 19

Heavy chains are interconnected by

Answer 19

DISULFIDE LINKAGES

Question 20

Ig has 2 terminal regions:

Answer 20

Carboxyterminal & Aminoterminal

Question 21

with constant amino acid sequence (constant region) AKA. Fc region

Answer 21

Carboxyterminal

Question 22

(Crystallizable Fragment/ Fragment crystalline)

Answer 22

Fc region

Question 23

with varying antibody specificity (variable region) AKA. Fab

Answer 23

Aminoterminal

Question 24

where epitope of antigen interacts

Answer 24

Fab - Fragment for Antigen Binding or Antigen Binding Fragment

Question 25

specific location where epitope of antigen interacts with the antibody

Answer 25

Hypervariable portion of Fab region

Question 26

Treatment of monomer antibody with enzyme papain (cleave covalent bonds) will produce 3 fragments:

Answer 26

One Fc fragment
Two Fab fragments (monovalent)

Question 27

fragment crystalline; involve in Ig biologic function

Answer 27

One Fc fragment

Question 28

Biologic functions of Fc fragment is involved in:

Answer 28

✓ Complement Fixation
✓ Placental Transfer of antibody
✓ Binding site for cell/s

Question 29

capable of antigen binding even without the Fc but cannot agglutinate or precipitate; separated 2 Fab fragments

Answer 29

Two Fab fragments (monovalent)

Question 30

Treatment with pepsin results in digestion of Fc fragment → destroyed → leaving 2 Fab fragments. The two Fab fragments have two antigens combining sites (Bivalent)

Answer 30

therefore, it is capable of antigen binding, precipitation and agglutination