Molecular Diagnositics Intro & History

Question 1

Definition:
-Detection of specific DNA or RNA molecules
-The study of nucleic acids and specific nucleic acid sequences

Answer 1

Molecular Diagnostics

Question 2

Discovered basic laws of inheritance by studying pea plants

Answer 2

Gregor Mendel

Question 3

Used pus from bandages for experiments and discovered nuclein (likely nucleic acids)

Answer 3

Frederick Miescher

Question 4

Used pathogenic and non-pathogenic pneumococci to develop his “transforming principle”

Answer 4

Frederick Griffith

Question 5

Trio of scientists that worked together to determine that DNA was required for transformation

Answer 5

-Oswald Avery
-Colin MacLeod
-Maclyn McCarthy

Question 6

Chemist that investigated DNA composition and discovered that there was a fixed ratio of bases.

Answer 6

Erwin Chargaff

Question 7

Chargaff’s rules

Answer 7

In any species, the ratio of A to T is 1:1 and G to C is 1:1.

Question 8

Chemist that was able to get high-resolution photos of crystallized DNA fibers to determine the basic dimensions of DNA strands and that the phosphates were on the outside of a helical structure

Answer 8

Rosalind Franklin

Question 9

Physicist produced x-ray diffraction images which, alonsgide Franklin’s images, helped determine the shape of DNA

Answer 9

Maurice Wilkins

Question 10

Scientists who proposed a 3D model of DNA in 1953. The original model was “inside out”.

Answer 10

James Watson and Frances Crick