Lab Exercise 4 (Aseptic Technique) Flashcards

1
Q

Define aseptic technique

A

working with microbes so that:
1) do not contaminate culture
2) don’t infect people

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2
Q

Describe how aseptic technique applies to different lab techniques (i.e.: handling plates or tubes, etc.)

A

Plates- burn inoculation loop while re-streaking
Tubes- burn the tube before putting lid on

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3
Q

What is a pure culture?

A

a culture with only 1 species

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4
Q

If given a plate could you tell if the culture was pure by just looking?

If given a broth culture could you tell if the culture was pure by just looking?

A

Trypticase soy agar (TSA) blue
Nutrient agar (NA) black

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5
Q

Know the appropriate techniques for labeling, incubation, and disposal of a petri plate

A

Name, Date, Class time, type of agar, what experiment?

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6
Q

Be able to describe the streak plate technique for isolated colonies.
If given a plate that was not successfully streaked, could you determine what went wrong?

A
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