Lab Exercise 4 (Aseptic Technique) Flashcards
Define aseptic technique
working with microbes so that:
1) do not contaminate culture
2) don’t infect people
Describe how aseptic technique applies to different lab techniques (i.e.: handling plates or tubes, etc.)
Plates- burn inoculation loop while re-streaking
Tubes- burn the tube before putting lid on
What is a pure culture?
a culture with only 1 species
If given a plate could you tell if the culture was pure by just looking?
If given a broth culture could you tell if the culture was pure by just looking?
Trypticase soy agar (TSA) blue
Nutrient agar (NA) black
Know the appropriate techniques for labeling, incubation, and disposal of a petri plate
Name, Date, Class time, type of agar, what experiment?
Be able to describe the streak plate technique for isolated colonies.
If given a plate that was not successfully streaked, could you determine what went wrong?