Lab Exercise 4 (Aseptic Technique)

Question 1

Define aseptic technique

Answer 1

working with microbes so that:
1) do not contaminate culture
2) don’t infect people

Question 2

Describe how aseptic technique applies to different lab techniques (i.e.: handling plates or tubes, etc.)

Answer 2

Plates- burn inoculation loop while re-streaking
Tubes- burn the tube before putting lid on

Question 3

What is a pure culture?

Answer 3

a culture with only 1 species

Question 4

If given a plate could you tell if the culture was pure by just looking?

If given a broth culture could you tell if the culture was pure by just looking?

Answer 4

Trypticase soy agar (TSA) blue
Nutrient agar (NA) black

Question 5

Know the appropriate techniques for labeling, incubation, and disposal of a petri plate

Answer 5

Name, Date, Class time, type of agar, what experiment?

Question 6

Be able to describe the streak plate technique for isolated colonies.
If given a plate that was not successfully streaked, could you determine what went wrong?