Lab Exercise 6A (Indirect Staining and Motility) Flashcards

1
Q

Know how to prepare a smear
Why do you do this?

A

Drop of water on slide then add cells, air dry, heat fix (pass X3 )
Kills and glues cells

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2
Q

What are the advantages and disadvantages to staining?

A

Increase contrast (color cell vs. color background)
makes structures visible
kills cells (they don’t move; can’t infect)

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3
Q

How do stains work?
What are they? Why do they stick to cells?

A
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4
Q

What’s the difference between a simple stain and a differential stain?

A

Simple Stain- one step, all cell types will be the same color
kk
Differential Stain- multiple steps, different cells will appear different even though they were all treated the same

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5
Q

What’s the difference between a direct stain and an indirect stain?

A

Direct Stain: stains the cells, white background / basic dye(alkaline) + ion (cation) is colored

Indirect Stain: Stains the background cells are white/ acidic dye - ion (anion) is colored

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6
Q

What are the 3 cell shapes and there arrangements

A

1) Cocci= Spherical
Diplococci (pairs)/ Streptococci (chains)/ Tetrad (four)
Staphylococcus (clusters)/ Sarcinae (cubical packet)/
Single cells
2) Bacilli= Rod-like or cylindrica
Diplobacilli (pairs)/ Streptobacilli (chains)/
Palisading (side-by-side)/ V-shaped/ Single Cells/
Coccobacilli (short rods)
3) Vibrio and Spirilla= curved
Vibrio (One- half spiral turn: Moon like)
Spirilla (full spiral turn)

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7
Q

Know how bacterial motility can be determined with laboratory methods.

A

DATA: Running & tumbling RESULT: + CONCLUSION: motile
No running & tumbling RESULT: - CONCLU. : not motile

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