Inhibition of Enzymes, Cofactors and Activation Energy Flashcards
Why do Enzyme Inhibitors exist?
To help slow or stop enzyme controlled reactions
What is the first type of Enzyme Inhibition?
Competitive Inhibitors
What is a competitive Inhibitor?
A substrate that acts as a competitor with the substrate
What type of bonding do Competitive inhibitors possess with enzyme’s active site?
Weak bonding
Is competitive inhibtion temporary of permanent?
Temporary
How does a competitive inhibitor prevent substrate from binding with Enzyme
Competitive inhibitor binds to active site of enzyme and physically blocks the substrate from binding
How does Competitive inhibition affect rate of reaction
Slows down rate of reaction, but will eventually reach Max Rate
Why does rate of reaction reach the max rate even though competitive inhibitors are present?
With very high [sub], competitive inhibition can be overcome as the chance a substrate will bind to active site iis almost guaranteed.
Competitive inhibition is also reversible as high [sub] can push inhibitor out of active site due to weak bonding
What is the second type of inhibtion called?
Non-competitive Inhibtion
Where does the non-competitive inhibitor bind?
Allosteric Site
What is an allosteric site?
A binding site on enzyme that is not the active site
How does a non-competitive inhibitor prevent substrate from binding with enzyme?
Non-competitive inhibitor binds to allosteric site and changes the enzyme’s active site shape, resulting in denaturing.
Is non-competitive inhibition temporary or permanent
Permanent
Discuss rate of reaction with presense of non-competitive inhibitors
Non-competitive inhibition does not reache the same maximum rate as no inhibition, as inhibitor binds to different site as substrate. Rate of reaction wil be lower.
What is a cofactor?
Non-protein substances that assist enzymes to function
