1-1 Flashcards
In a blocking ELISA for testing of antibody:
enzyme labelled antibody is added after the first incubation with the serum- containing antibody
The zone of equivalence is a place, where:
the concentration of antigen is in balance with the concentration of antibodies
The wavelength used for spectrometric analysis of ELISA tests is dependent on the used:
substrate
The preparation of hybridomas requires:
B-lymphocytes from the spleen of immunized mouse and myeloma cells.
The essential step for Western blot diagnosis of BSE is:
treatment of the sample with Proteinase K.
Gammopathies are diseases characterized by:
overproduction of defective immunoglobulins
In electrophoresis, individual molecules are separated depending on their:
total electric charge and molecular mass.
A positive reaction in a tube agglutination test is:
white sediment (with the shape of a dot) on the bottom and a clear supernatant.
The precipitin band has/is:
A white colour.
Fill in the blanks in the following statements with the most appropriate expression.
Blood [ ] is prepared by centrifugation of the clotted blood.
Blood [ ] is prepared by centrifugation of whole blood.
Blood [serum] is prepared by centrifugation of the clotted blood.
Blood [plasma] is prepared by centrifugation of whole blood.
which of the following agglutination assays is for quantitative determination of antibodies ?
Haemagglutination inhibition test.
Tube agglutination.
Prozone is :
inability to cause agglutination in excess of antibodies.
