1:2 Flashcards
Protein A can bind to:
the Fc region of the antibody
After serum electrophoresis, immunoglobulins are present in the fraction of:
gamma globulins.
The result of a Western blot is evaluated as a change of colour:
on the NC membrane.
SDS (sodium dodecyl sulphate) is used for the preparation of samples for PAGE because of:
gives the same negative charge to all proteins.
‘Serum inactivation is used to:
functionally inactivates the complement system.
In the sandwich ELISA they have:
labelled antibodies have the same specificity as solid-phase bound antibodies.
Which of the following immunoelectrophoresis is for determination of gammopathies in blood serum?
Classical immunoelectrophoresis.
Fill in the blanks in the following statements with the most appropriate expression.
Whole blood is used to determine immunoglobulin concentrations using a […..] test.
Blood serum is used to determine immunoglobulin concentrations using a […… ] test.
Whole blood is used to determine immunoglobulin concentrations using a [glutaraldehyde] test.
Blood serum is used to determine immunoglobulin concentrations using a [zinc sulphate ] test.
A negative reaction in a tube agglutination test is:
white sediment (with the shape of a dot) on the bottom and a turbit supernatant.
In an indirect ELISA for testing of antibody, the intensity of colour measured after adding the substrate in the final step is directly proportional to the concentration of:
antibodies in the patient serum.
The speed of the movement of molecules during electrophoresis is dependent on:
molecular mass.
Agarose gel is prepared by:
boiling of agarose suspension.
