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Question 1

In a sandwich ELISA:

Answer 1

The labelled antibody is not of identical specificity as the antibody bound to the solid phase

Question 2

For competitive ELISA is NOT used the step:

Answer 2

The antigen-antibody mixture is added to an antigen coated well

Question 3

In a blocking ELISA:

Answer 3

Enzyme labelled antibody is added after the 1st incubation with the serum containing antibody

Question 4

Which of the following components is for ELISA:

Answer 4

Antibody labelled with enzyme

Question 5

Which of the following enzymes is NOT for enzymoimmunoassays:

Answer 5

• Myeloperoxidase
• Beta-galactosidase

Question 6

Which of the following components is NOT substrate for enzymoimmunoassays:

Answer 6

Fluorescein

Question 7

What is conjugate

Answer 7

Antibody labelled with enzyme

Question 8

Which of the following components is for IFA:

Answer 8

Antibody labelled with fluorochrome

Question 9

The indirect IFA is most commonly used for:

Answer 9

Testing of autoimmune disorders

Question 10

Flow cytometry is used for:

Answer 10

Analysis of the cells based on their differential ability to bind fluorescence labelled antibody

Question 11

Which of the following immunoblotting assays is for detection of protein:

Answer 11

Western blotting

Question 12

The essential steps of Western blotting are:

Answer 12

BSEElectrophoresis, transfer and immunostaining