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Molecular Microbiology > Cell division > Flashcards

Cell division Flashcards

1
Q

Asynchronous cultures

A

Culture with cells at different points of the cell cycle

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2
Q

Synchronous cells

A

Culture with cells at the same points in the cell cycle
Ideal for studying

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3
Q

Induction methods

A
  • Growth after nutrient starvation
  • Change in temperature
  • Growth inhibitor

significant metabolic disturbance but high cell yield

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4
Q

Selection methods

A

Size
Age

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5
Q

Baby machine

A

Selection by age mechanism
Cells divide into funnel
Collected at intervals - same age and therefore synchronous

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6
Q

Conditionally lethal mutants

A

Mutants that can grow under permissive conditions but die under restrictive conditions e.g. temp sensitive mutants

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7
Q

System 1 genes

A

Involved in increasing cell size

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8
Q

System 2 genes

A

Involved in creating cylindrical rod shape

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9
Q

System 3 genes

A

Involved in cell division w formation of new septum

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10
Q

Diffuse intercalation

A

Gram positive: Cell gets larger by additional of new cell wall material all over cell wall

Gram negative: E.coli by putting materially equally in interior of cell wall = expansion of cell

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11
Q

FtsZ

A

Prokaryotic homologue of eukaryotic tubulin
GTPase

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12
Q

Divisome

A

FtsZ + other proteins e.g. ZipA, Ftsl that cause cell separation

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13
Q

ZipA

A

Anchors FtsZ to cell membrane

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14
Q

FtsA

A

ATPase - depolarisation + constriction of z-ring

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15
Q

FtsI

A

Transpeptidase involved in crosslinkng PG to cell wall

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16
Q

FtsK

A

Separates chromosomes

17
Q

How is septum location controlled?

A
18
Q

Min proteins

A
19
Q

Dichotomous/multi fork replication

A
20
Q

DNA-A

A

Replication initiator
binds to ATP for active form and then binds to DNA-A box

21
Q

DNA-A box

A

AT rich region to which DNA-A binds to
Facilitates unwinding of DNA

22
Q

Frequency of replication is controlled by…?

A

The availability of DNA-A in ATP form

23
Q

RIDA

A

Regulatory Inactivation of DNA-A

24
Q

DAM

A

DNA adenine methylase
Methylates A and recognises GATC domains in oriC

25
Q

SeqA

A

Membrane bound protein
Binds to new hemimethylated sequencr
Pulls apart chromosomes when cell divides

Molecular Microbiology (19 decks)

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