Culturing microbes in a streak plate and inhibition zone

Question 1

What is an aseptic technique?

Answer 1

= working in sterile conditions
= prevents contamination and infection of others

Question 2

What is pre-inoculation?

Answer 2

= sterilize all equipment to kill microcrobes
= sterilize work surfaces with desinfeftent to kill microves
= wash hands with soap to remove microbes

Question 3

what is inoculating?

Answer 3

= work near a bunsen burner- sterilises air
= only open petri dish lid slightly when necessaeru
= flame neck of any bottles used before and after

Question 4

What is post inoculation?

Answer 4

= sterilize all equipment to kill microcobes
= sterilize work surfaces with disenfectant
= wash hands with soap

Question 5

What is the method?

Answer 5

= wash hands
= googles must be worn
= sterilize work surface with vekron, light bunsen burner into roaring flame
= transfer bacteria from broth to agar plate
= work near the bunsen burner streak the plate evenly distributed using a sterile plastic spreader as quick as possible with tilted lid and put the lid on
= place a multi disc antibotic ring on the plate with sterile forceps-pass through bunsen burner flame (has different antibiotucs)
= tape the lid lightly- so it does not fall off and don’t tape the whole way as we want oxygem to get in
= invert the plate and incubate 25 degrees for 48 hours
= after incubation, for each antibotic, measure the diamater of the inhbitition zone, clear circle
= calculate area of the inhbitiion zone

Question 6

What do you label the petri dish?

Answer 6

= initials
= date done
= contents

Question 7

What equation is used?

Answer 7

= area= pi x diameter/4

Question 8

What is the risk and control of a naked flame of the bunsen burner?

Answer 8

= burn- fire hazard
= control: tie up long hair, wear goggles, keep away from flammable items,

Question 9

What is the risk and control of bacteria?

Answer 9

= contamination, leads to infection
= wash hands before and after, dont open plate after incubation, use disinfectant before and after, incuvate at 25 degrees

Question 10

What is the risk and control of disinfectant?

Answer 10

= flammable
= keep away from naked flame