To investigate the effect of temperature on enzyme action Flashcards

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1
Q

Apparatus

A
  • 3 graduated cylinders with celery
  • pH buffer 7 & 1 drop washing up liquid
    (in 3 seperate water baths at 0ºC, 25ºC & 80ºC)
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2
Q

Steps

A
  • Add Hydrogen peroxide to all 3 cylinders
  • Vol in graduated cylinders noted at 0,1&2 mins
  • Rate of enzyme action calculated by 2min vol - 1min vol
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3
Q

Result

A

25ºC graduated cylinder showed the most enzyme action

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