To mobilise an enzyme

Question 1

Steps to immobilise enzyme

Answer 1

• Yeast cells immobilised using sodium alginate
• Yeast suspension mixed w/sodium alginate solution
• dropeed into calcium chloride to solidify
• Beads of alginate washed (x3) & placed in dropping funnel

Question 2

Steps to examining its application

Answer 2

• Sucrose solution placed into funnel & immobilised yeast acts on it
• Product released by opening tap
• Product tested for reducing sugar using glucose test strips

Question 3

Result

Answer 3

Immobilised yeast converted the sucrose to glucose+fructose without contaminating product