Mounting/Coverslipping

Question 1

Purpose of mounting

Answer 1

To protect tissue from damage/injury
To prevent bleaching of sections
For permanent safekeeping and to facilitate easy
handling and storage

Question 2

Mounting medium should have a refractive index close to that of the slide

Answer 2

1.518

Question 3

Last step in tissue processing

Answer 3

Labelling

Question 4

syrupy fluid applied
between the tissue and the coverslip

Answer 4

Mouning medium

Question 5

used for preps that have been dehydrated and cleared with xylene; permanent mounting media

Answer 5

Resinous mountant

Question 6

Canada balsam refractive index

Answer 6

1.524

Question 7

refractive index DPX

Answer 7

1.532

Question 8

XAM refractive index

Answer 8

1.52

Question 9

CLARITE refractive index

Answer 9

1.544

Question 10

designed to mount water miscible preparations;
temporary mountants

Answer 10

Aqueous mountant

Question 11

Refractive index of Glycerin Jelly

Answer 11

1.47

Question 12

Refractive index of Farrant’s/ Gum arabic

Answer 12

1.43

Question 13

Refractive index of apathy’s - methylene blue stained nerve

Answer 13

1.52

Question 14

Mounting frozen sections directly from water LMPA

Answer 14

Brun’s fluid

Question 15

not used in practice Disadvantage: low
refractive index and evaporates easily, does not allow examination of tissues under OIO

Answer 15

Water

Question 16

Process of sealing the margins of the cover slip

Answer 16

Ringing

Question 17

Purpose of ringing

Answer 17

To prevent escape of mounting medium/ fluid
Prevent sticking of slides upon storage and immobilize
To immobilize the coverslip
To prevent evaporation of mountant

Question 18

Used for ringing

Answer 18

Kronig cement and Durofix

Question 19

Indicating year and specimen number for proper
identification

Answer 19

Labeling

Question 20

NEUTRAL/AMPHOTERIC DYE

Answer 20

Giemsa, Leishman, Irishman

Question 21

SOLVENTS USED FOR STAINS (WAPA)

Answer 21

Water
Alcohol (ethyl or methyl)
Phenol
Aniline water

Question 22

Process of coating slide with thin celloidin solutions
Done if sections will be subjected to strong alkali or
acid solutions and for tissues containing glycogen for
demonstration

Answer 22

COLLODIONIZATION

Question 23

RE-STAINING OF OLD SECTIONS

Answer 23

1. Immersion in xylene for 24 hrs: to facilitate removal of the cover slip
2. Removal of coverslip
3. Immersion in xylene 30 minutes: to further remove the mountant
4. Immersion in 0.5% potassium permanganate 5 10 minutes, rinse with tap water
5. Immersion in 5% oxalic—to remove the previous stain; bleaching agent
6. Re-stain

Question 24

RETENTION PERIOD Reports: 10 years

Answer 24

Clinical pathology laboratory reports
Surgical pathology and bone marrow Reports

Question 25

RETENTION PERIOD specimens: 10 years

Answer 25

Pathology /Bone marrow slides
Pathology block

Question 26

RETENTION PERIOD: indefinite

Answer 26

Autopsy forensic reports

Question 27

RETENTION PERIOD records: 20 years

Answer 27

Cytogenetic report

Question 28

RETENTION PERIOD specimen: 3 years

Answer 28

cytogenetic slides

Question 29

RETENTION PERIOD spx: 20 years

Answer 29

Cytogenetic diagnostic images

Question 30

substitute for mercuric chloride

Answer 30

zinc sulfate

Question 31

Factors to be considered when choosing the right
decalcifier

Answer 31

Urgency of the case (fast-acting)
Staining technique to be used
Degree of mineralization (calcium content, if high- strong decalcifier is used)

Question 32

Fixative to be avoided prior to decalcification

Answer 32

Carnoy’s fluid

Question 33

Factor’s affecting decalcification rate

Answer 33

Tissue structure
Size and consistency of specimen
Temperature (18-30°C)
Volume and concentration

Question 34

Types of Autotechnicon

Answer 34

Tissue transfer type/ Dip and dunk
Fluid transfer type/ Enclosed type