producing DNA fragments using the gene machine

Question 1

define biosafety

Answer 1

aims to protect public health and environment from accidental exposure to biological agents

Question 2

define biosecurity

Answer 2

deals with the prevention of miuse through loss,theft,diversion, intentional release of pathogens

Question 3

what are the advantages of using a gene machine

Answer 3

+ quick
+ no introns (prokaryotes cannot splice)
+ any sequence of nucleotides
+ accurate

Question 4

what are the 3 ways to isolate gene fragments

Answer 4

• reverse transcriptase
• restriction endonucleases
• gene machine

Question 5

explain how gene machine is used to produce DNA fragments

Answer 5

• desired protein
• amino acid sequence
• mRNA sequence
• DNA sequence
• nucleotide base sequence into a computer
  -biosafety/biosecurity/international standards/ethics check
• computer designs oligonucleotides (small, overlapping, single stranded nucleotides)
• oligonucleotides assembled one nucleotide at a time
  (automated)
• oligonucleotides joined to form gene (all coding DNA. single strand)
• PCR replicates gene
  (makes complementary strand for DNA first)
• gene into plasmid using sticky ends (vector for storage/cloning/transfer)
• genes
  check using sequencing techniques