(Laboratory) Microscopy Flashcards

Microscopy

1
Q

Micron (μ) and Millimicron (mμ) have been replaced by the terms _____ and _____

A

Micrometer (μm) and Nanometer (nm)

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2
Q

_____ and _____ have been replaced by the terms micrometer (μm) and nanometer (nm)

A

Micron (μ) and Millimicron (mμ)

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3
Q

Sizes of bacteria, fungi, and unicellular parasites are usually expressed in terms of _____

A

Micrometers

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4
Q

Microbes are measured using an _____

A

Ocular Micrometer

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5
Q

It is a tiny ruler within the eyepiece (ocular) of the compound light microscope

A

Ocular Micrometer

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6
Q

1 meter is equivalent to _____ centimeters

A

100

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7
Q

1 centimeter is equivalent to _____ millimeters

A

10

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8
Q

1 millimeter is equivalent to _____ micrometer

A

1,000

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9
Q

Approximate size of most viruses

A

0.01 to 0.3 micrometer

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10
Q

Approximate size of Cocci bacteria

A

1 micrometer

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11
Q

Approximate size of Bacilli bacteria

A

1 micrometer

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12
Q

It is a spherical bacteria

A

Cocci

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13
Q

Types of Bacteria according to its shape

A

Cocci
Bacilli

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14
Q

Approximate size of Yeast

A

3 to 5 micrometer

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15
Q

It is a rod-shaped bacteria

A

Bacilli

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16
Q

Approximate size of a Septate hyphae

A

2 to 15 micrometer

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17
Q

Approximate size of an Aseptate hyphae

A

10 to 30 micrometer

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18
Q

A hyphae containing cross-walls

A

Septate hyphae

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19
Q

A hyphae without cross-walls

A

Aseptate hyphae

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20
Q

List down 3 Fungi:

A

Yeast
Septate hyphae
Aseptate hyphae

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21
Q

Approximate size of Chlamydomonas

A

5 to 12 micrometer

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22
Q

Approximate size of Euglena

A

35 to 55 micrometer

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23
Q

Approximate size of Vorticella

A

50 to 145 micrometer

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24
Q

Approximate size of Paramecium

A

180 to 300 micrometer

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25
Q

Approximate size of Volvoxa

A

350-500 micrometer

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26
Q

Approximate size of Stentor

A

1,000 to 2,000 micrometer

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27
Q

List down 6 types of Pond Water Protozoa

A

Chalmydomonas
Euglena
Vorticella
Paramecium
Volvoxa
Stentor

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28
Q

A microscope that has only one magnifying lens

A

Simple Microscope

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29
Q

This is an example of a Simple Microscope

A

Magnifying Glass

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30
Q

In Simple Microscopes, images appear about _____ larger than the object’s actual size

A

3 to 20 times

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31
Q

In Simple Microscope, images appear about 3 to 20 times (smaller or larger) than the object’s actual size

A

Larger

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32
Q

Leeuwenhoek’s simple microscopes had a maximum magnifying power of about _____

A

300 times

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33
Q

_____ simple microscopes had a maximum magnifying power of about X300 (300 times)

A

Leeuwenhoek’s

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34
Q

A microscope that contains more than one magnifying lens

A

Compound Microscope

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35
Q

Who are given credit for being the first to conceptualize Compound Microscopes?

A

Hans Jansen
Zacharias (Hans Jansen’s Son)

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36
Q

Compound Microscopes magnify objects about _____

A

1,000 times

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37
Q

Photographs taken through the lens system of Compound Microscopes are called _____

A

Photomicrographs

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38
Q

Compound Microscopes is also referred to as _____

A

Compound Light Microscope

39
Q

In Compound Microscopes, the wavelength of visible light is approximately _____ that limits the size of objects that can be seen

A

0.45 micrometer

40
Q

Part of the Microscope that is located at the top of the microscope

A

Ocular Lens

41
Q

Part of the Microscope that is located above the stage

A

Revolving Nosepiece

42
Q

Part of the Microscope that is held in place above the stage by the revolving nosepiece

A

Objective Lenses

43
Q

Part of the Microscope that is directly beneath the nosepiece and objective lenses

A

Stage

44
Q

Part of the Microscope that is beneath the stage

A

Stage Adjustment Knobs

45
Q

Part of the Microscope that is located on the condenser

A

Iris Diaphragm Control Arm

46
Q

Part of the Microscope that is located beneath the stage

A

Condenser

47
Q

Part of the Microscope that is located beneath the condenser

A

Collector Lens with Field Diaphragm

48
Q

Part of the Microscope that is located on the front side of the base

A

Rheostat Control Knob

49
Q

Part of the Microscope that is attached to the field diaphragm

A

Field Diaphragm Lever

50
Q

Part of the Microscope that is on the side of the base

A

On and Off Switch

51
Q

Part of the Microscope that is located beneath and behind the condenser

A

Condenser Control Knob

52
Q

Part of the Microscope that is located on the arm of the microscope near the base

A

Fine and Coarse Adjustment Knobs

53
Q

What is the part of the Microscope that holds the objective lenses?

A

Revolving Nose Piece

54
Q

What is the part of the Microscope that is used to magnify objects placed on the stage?

A

Objective Lenses

55
Q

What is the part of the Microscope that has a flat surface on which the specimen is placed for viewing?

A

Stage

56
Q

What is the part of the Microscope that is used to move the stage and microscope slide?

A

Stage Adjustment Knobs

57
Q

What is the part of the Microscope that is used to adjust the amount of light passing?

A

Iris Diaphragm Control Arm

58
Q

What is the part of the Microscope that contains a lens system that focuses light onto the specimen?

A

Condenser

59
Q

What is the part of the Microscope that controls the amount of light entering the condenser source?

A

Collector Lens with Field Diaphragm

60
Q

What is the part of the Microscope that controls the amount of light emitted from the light?

A

Rheostat Control Knob

61
Q

What is the part of the Microscope that is used to adjust the amount of light passing through the collector lens?

A

Field Diaphragm Lever

62
Q

What is the part of the Microscope that is used to turn the light source on and off?

A

On and Off Switch

63
Q

What is the part of the Microscope that contains the light source?

A

Base

64
Q

What is the part of the Microscope that is used to adjust the height of the condenser?

A

Condenser Control Knob

65
Q

What is the part of the Microscope that is used to focus the objective lenses?

A

Fine and Coarse Adjustment Knobs

66
Q

What is the part of the Microscope that supports the binocular body and the revolving nosepiece; held with one hand when carrying the microscope, with the other hand beneath the base to support the weight of the Microscope?

A

Arm

67
Q

What is the part of the Microscope that holds the ocular lenses in their proper locations?

A

Binocular Body

68
Q

A microscope that is often used in the Clinical Laboratory

A

Brightfield Microscope

69
Q

A microscope in which the objects are observed against a bright background

A

Brightfield Microscope

70
Q

A microscope in which the objects are observed or seen against a dark background

A

Darkfield Microscope

71
Q

This is a Microscope that is used in the diagnosis of Syphilis

A

Darkfield Microscope

72
Q

In a Darkfield Microscope, the condenser is replaced with what is known as _____

A

Darkfield Condenser

73
Q

Magnification of a Scanning Objective Lens

A

4x

74
Q

Magnification of a Low Power Objective Lens

A

10x

75
Q

Magnification of a High Power Objective Lens

A

40x

76
Q

Magnification of an Oil Immersion Objective Lens

A

100x

77
Q

A Microscope that is used to observe unstained living microorganisms

A

Phase-contrast Microscope

78
Q

A Microscope that is often used in Immunology Laboratories

A

Fluorescence Microscope

79
Q

Fluorescence Microscopes contains a built-in _____

A

Ultraviolet Light Source

80
Q

A Microscope that is capable to view viruses

A

Electron Microscope

81
Q

Electron Microscope uses an _____ as a source of illumination and magnets to focus the beam

A

Electron Beam

82
Q

Electron Microscope uses and electron beam as a source of illumination and _____ to focus the beam

A

Magnets

83
Q

The wavelength of electrons traveling in a vacuum is much (longer or shorter) than the wavelength of visible light

A

Shorter

84
Q

Two types of Electron Microscope

A

Transmission Electron Microscopes
Scanning Electron Microscopes

85
Q

A Microscope that is used to view the inside of your cell; inferior of the cell

A

Transmission Electron Microscope

86
Q

In Transmission Electron Microscope, specimen must be very _____ and the electron beam will pass through the specimen

A

Thin

87
Q

In Transmission Electron Microscope, electron gun fires a beam of electrons _____

A

Downward

88
Q

A Microscope that is used to observe the outer surfaces of specimens

A

Scanning Electron Microscope

89
Q

Scanning Electron Microscope’s photographs are called _____

A

Scanning Electron Micrographs

90
Q

Transmission Electron Microscope’s photographs are called _____

A

Transmission Electron Micrographs

91
Q

Most powerful type of Microscope

A

Atomic Force Microscope

92
Q

A Microscope that observe living cells at extremely high magnification and resolution

A

Atomic Force Microscope

93
Q

Formula for Total Magnification

A

Eyepice x Objective