3. Blood groups I Flashcards

1
Q

ABO common precursor molecule

A

H

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2
Q

type 1H

A

soluble antigens present in secretions and plasma

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3
Q

type 2H

A

antigens present on RBCs

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4
Q

H antigen structure includes an ——– added onto the terminal galactose

A

L-fucose

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5
Q

O transferase

A

Fucosyltransferase

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6
Q

A transferase

A

Acetylgalactosaminyltransferase

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7
Q

B transferase

A

Galactosyltransferase

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8
Q

A sugar

A

N-acetylgalactosamine

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9
Q

B sugar

A

D-galactose

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10
Q

Expression of ABO alleles depends on the —- gene

A

H

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11
Q

naturally-occurring IgG, reacts with both type A and type B cells

A

anti-A,B
found in type O people

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12
Q

clinical significance of anti-A,B

A

able to cross placenta and cause mild HDFN

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13
Q

Tests for ABH secretions may establish true group when…

A

RBC antigens are poorly developed/absent

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14
Q

+ reaction with subgroup A1 cells

A

Dolichos biflorus

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15
Q

used to subgroup ABOs based on amount of H present

A

Ulex europaeus (H lectin)

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16
Q

most H –> least H among subgroups

A

O → A2 → B → A2B → A1 → A1B

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17
Q

hh

A

Bombay

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18
Q

antibodies present in plasma of Bombays

A

anti-A, anti-B, anti-A,B, and anti-H

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19
Q

homozygous hh inherited

A

classical Bombay

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20
Q

hh, Se

A

Para-Bombay

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21
Q

Lewis discovery

A

Mourant in 1946

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22
Q

Le gives fucosyltransferase which adds an L-fucose onto…

A

N-acetylglucosamine

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23
Q

Le are expressed on ———– adsorbed onto RBCs

A

glycosphingolipids

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24
Q

newborn Le phenotype

A

Le(a=b=)

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25
Q

Le + Se phenotype < 6yo

A

Le(a+b+)

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26
Q

Se gene adds L-fucose onto…

A

terminal galacose

27
Q

Le, sese, H

A

secretions: Lea

RBC: Le(a+b=)

28
Q

Le, Se, H

A

secretions: Lea, Leb, H

RBC: Le(a=b+)

29
Q

lele, sese, H

A

secretions: none

RBC: Le(a=b=)

30
Q

lele, Se, H

A

secretions: H

RBC: Le(a=b=)

31
Q

Le, sese, hh

A

secretions: Lea

RBC: Le(a+b=)

32
Q

Le, Se, hh

A

secretions: Lea, Leb, H

RBC: Le(a=b+)

33
Q

lele, sese, hh

A

secretions: none

RBC: Le(a=b=)

34
Q

lele, Se, hh

A

secretions: H

RBC: Le(a=b=)

35
Q

most common Rh phenotypes in white people

A

1) R1 or DCe
2) r or dce
3) R2 or DcE
4) R0 or Dce

36
Q

most common Rh phenotypes in Black people

A

1) R0 or Dce
2) r or dce
3) R1 or DCe
4) R2 or DcE

37
Q

Made from pools of human sera
20-24% protein

A

high protein Rh antisera

38
Q

Monoclonal IgM suspended in saline

A

saline Rh antisera

39
Q

Human IgG with opened S-S bonds, allowing the molecule to span greater distances

A

chemically modified IgG Rh antisera

40
Q

Monoclonal IgM blended with polyclonal IgG with a protein concentration = to normal human serum

A

monoclonal/polyclonal Rh antisera

41
Q

pros of high protein antisera

A

rapid; reliable; protein enhances aggregation

42
Q

cons of high protein antisera

A

false + with Ab-coated RBCs; must run Rh control; may cause rouleaux; cannot detect weak-D

43
Q

pros of saline antisera

A

avoids rouleaux and spontaneous agglutination

44
Q

cons of saline antisera

A

less sensitive; not suitable for weak-D

45
Q

pros of chemically modified antisera

A

more sensitive; less rouleaux; no control necessary

46
Q

cons of chemically modified antisera

A

expensive

47
Q

pros of monoclonal/polyclonal antisera

A

IgM works for IS, IgG works for weak-D; little rouleaux; most partial antigens detected

48
Q

weak-D test procedure

A

Negative D test at IS…

Incubate at 37° for 15 minutes
Wash and add AHG
A positive indicates weak-D
(Checkcells are used for QC on the AHG if the test is negative)

49
Q

3 weak-D variations

A
  • position effect (trans to C)
  • gene variation (weakened antigens)
  • mosaic/partial-D (missing epitopes)
50
Q

only test for anti-e if donor/patient is…

A

E+

51
Q

compound antigen

A

two genes that produce a unique product when present in the cis-position on a chromosome

52
Q

c + e in cis position

A

f compound antigen

53
Q

allelic variants

A

genes which vary from the common allele by only a few AAs (missing, additional, or misaligned)

54
Q

variant form of C seen in 2% of whites

A

CW

55
Q

serine residue at position 103 on 2nd extracellular loop of each protein (Exon 2)

A

G antigen

56
Q

anti-G is seen with…

A

anti-C

57
Q

prenatal patients with anti-G and anti-C can make…

A

anti-D

58
Q

how to test for anti-D in somebody with anti-G

A

remove anti-C/anti-G from plasma by adsorption onto D=, C+, G+ cells, leaving only anti-D in plasma if it’s present

59
Q

LW is a —— molecule

A

Intracellular adhesion molecule

60
Q

differentiate anti-LW from anti-D

A

Reacts weakly with Rh= cells (anti-D is NR)
Nonreactive with DTT-treated RBCs (anti-D is R)
Nonreactive with pronase-treated RBCs (anti-D is R)

61
Q

no Rh antigens

A

Rhnull

62
Q

Rmod

A

inheritance of modifier gene XQ, resulting in markedly reduced amounts of antigen

63
Q

2 ways to inherit Rhnull

A

Inheritance of 2 amorphic genes at Rh locus
Inheritance of suppressor genes at a regulator locus

64
Q

abnormalities associated with Rnull (5)

A

Stomatocytosis
Hemolytic anemia
Increased reticulocytes
Reduced RBC survival
Weakened expression of related antigens (Duffy, S, s, U, LW)