The Direct and Indirect Antiglobulin Tests

Question 1

Principle of the Antiglobulin Test

Answer 1

-uses antihuman globulin reagents to bind to human IgG or complement molecules that are sensitized to antigens on adjacent RBCs and thus form observable agglutination

Question 2

Antihuman globulin reagents (AHG)

Answer 2

-reagents with antibodies to human globulins such as IgG or complement, either free in the serum or attached to antigens on the RBCs

Question 3

Polyspecific AHG reagent

Answer 3

-contains antibodies to human IgG as well as to human complement components

Question 4

monospecific AHG reagent

Answer 4

-contain only one antibody specificity of either the Anti-Igg or Anti-Complement type