14. Molecular Diagnostic Tools 1 Flashcards
1
Q
What is gel electrophoresis?
A
Method of separating and analysing DNA, RNA and proteins based on their size and charge
2
Q
What is Western blotting used for?
A
Detect a specific protein in a sample
3
Q
What is the polymerase chain reaction used for?
A
To ‘photocopy’ DNA
4
Q
What is karyotyping used for?
A
Determine number and banding pattern of chromosomes
5
Q
What are the steps in karyotyping?
A
Cells are stimulated to divide Stopped in metaphase Burst open with hypotonic solution Drop cells onto microscope slide Giemsa staining Examine via microscope
