LESSON 11: ASEPTIC TECHNIQUE

Question 1

Is to eliminate contaminants from
work surfaces, solutions and other sources in the laboratory.
Establishing a strict
code of practice and adhere to it, particularly if several people share the same
workspace.

Answer 1

Aseptic technique

Question 2

Contamination can be avoided by
(1) regular checking of culture,
(2) avoidance of use of antibiotics for at least a part of incubation period to reveal cryptic
contaminations,
(3) always use sterile reagents and materials,
(4) exclusive use of
media, materials for different cell lines, and
(5) strict observation of standard sterile
technique at all

Question 3

Elements of aseptic environment

Answer 3

1. Quiet Place
2. Work Surface
3. Personal Hygiene
4. Reagent and media
5. Cultures

Question 4

STERILE HANDLING

Answer 4

1. SWABING
2. CAPPING
3. FLAMING
4. HANDLING BOTTLES AND FLASK
5. PIPETTING
6. POURING

Question 5

equipment that protect work surface from dust and contamination by a
constant, stable flow of filtered air passing over the work surface

Answer 5

SAFETY CABINET (LAMINAR FLOAW)

Question 6

Two types:

Answer 6

HORIZONTAL AND VERTICAL

Question 7

Where air blows from the side facing you, parallel to the work surface, and
not circulated.

Question 8

where air blows down from the top of the hood onto the work surface and is drawn through the work surface and either recirculated or vented

Question 9

are used to sterilize the air and exposed work surfaces in laminar
flow hoods between uses.

Answer 9

Ultraviolet light

Question 10

major source of contamination. Incubators should
be regularly cleaned with detergent and then swabbed with 70% alcohol and cleared
of any inappropriate contents.

Answer 10

Humidified incubator

Question 11

at the bottom of the incubator can
be supplemented with a fungicide to retard fungal growth

Answer 11

humidifier tray