MICRO MANUAL CHAPTER 1 & 2 Flashcards
what is biosafety in laboratorry?
Biosafety indicates about handling or reduce the risk in laboratory such biological agent and infectious.
Biosafety categorized as BSL 1 - 4, which each has a specific on how to control for contamination of microbes or agents during laboratory.
organisms are well-characterized strains of microorganisms
not known to cause disease in healthy human adults. Precautions in BSL-1 labs include
general laboratory safety.
Biosafety Level 1
organisms are moderate-risk microorganisms associated with
less serious human diseases whose potential for transmission is limited and a proven
treatment for the disease exists.
BSL 2
organisms are high-risk microorganisms with a true potential
for infection by aerosols and in which the resulting disease may have serious or lethal
consequences.
BSL 3
organisms are high-risk microorganisms with a true potential
for infection by aerosols and in which the resulting disease may have serious or lethal
consequences.
organisms are easily transmitted, very-high risk
microorganisms which cause life-threatening diseases for which there is no vaccine or
therapy.
How to conduct risk assessment?
- Identification of the hazards and risks:
- Risk evaluation:
- Risk mitigation plan
- Evaluation of effectiveness of control
1 (no or low individual and community risk): A microorganism that is unlikely
to cause human or animal
RISK GROUP 1
(moderate individual risk, low community risk):
A pathogen that can cause
human or animal disease but is unlikely to be a serious hazard to laboratory workers, the
community, livestock or the environment.
Laboratory exposures may cause serious
infection, but effective treatment and preventive measures are available and the risk of
spread
RISK GROUP 2
(high individual risk, low community risk): A pathogen that usually causes
serious human or animal disease but does not ordinarily spread from one infected
individual to another. Effective treatment and preventive measures are available
RISK GROUP 3
(high individual and community risk): A pathogen that usually causes
serious human or animal disease and that can be readily transmitted from one individual to
another, directly or indirectly. Effective treatment and preventive measures are not usually
available
GROUP 4
difference between hazard and risk
hazard can potentially cause a harm while risk is the chance that hazard can cause a harm.
Biosafety cabinet (BSC)
an enclosed, ventilated laboratory workspace for safely working with
materials contaminated with (or potentially contaminated with) pathogens requiring a
defined biosafety level.
safety cabinet with front opening through which the operator can carry out
manipulations inside the cabinet and which is constructed so that the worker is protected
and the escape of airborne particulate contamination generated within the cabinet is
controlled by means of inward airflow through the working front opening and filtration of
the exhaust air.
class 1
safety cabinet with front opening through which the operator can carry out
manipulations inside the cabinet and which is constructed so that the worker is protected, the risk of product and cross-contamination is low and the escape of airborne particulate
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contamination generated within the cabinet is controlled by means of an appropriate
filtered internal airflow and filtration of the exhaust air (laminar airflow)
class 2
: Safety cabinet in which the working area is totally enclosed and the operator is
separated from the work by a physical barrier (I.e. gloves mechanically attached to the
cabinet). Filtered air is continuously supplied to the cabinet and the exhaust air is treated to
prevent release of micro-organisms.
class 3
(to examine microorganisms which can’t
be seen by naked eyes)
microscope
(to heat or boil solution in laboratory)
- Bunsen burner (
(to sterilize the equipment medias and other
solutions)
Autoclave
(to sterilize the equipment medias
and other solutions)
Pressure cooker
