Meleson and Stahl Flashcards

1
Q

what were they trying to prove in this experiment

A

that dna is replicated semi conservatively

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2
Q

what did their experiemnt use.
what of

A

isotopes of nitrogen

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3
Q

what was the light nitrogen

A

14
N

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4
Q

what was the heavy nitrogen

A

15
N

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5
Q

what is an isotope

A

same number of protons and electrons but different number of neutrons

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6
Q

what are both spun in

A

a centrifuge

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7
Q

how many generations were there

A

4

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8
Q

what bacteria was grown in the medium containing 15N (g0)

A

100% of dna was 15N 15N

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9
Q

what bacteria was grown in the medium containing 14N (g1)

A

100% of dna was 15N 14N

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10
Q

what bacteria was grown in the medium containing 14N (g2)

A

50% of dna was 14N 14N
50% of dna was 15N 14N

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11
Q

what bacteria was grown in the medium containing 14N

A

75% of dna was 14N 14N
25% of dna was 15N 14N

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12
Q

how do these reults prove dna is replicted using the semi conservatve method

A

because they found that dna was found only in the middle of the test tube after centrifugation.

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