Blood Bank Makeup Exam Part 1 Flashcards
At birth, infant cells are rich in ____ and ___ is nearly undetectable
I/i
None of the above
i/I
I/H
i/I
How is a weakly expressed D antigen detected?
Treating the cells with 2-ME
A positive indirect antiglobulin test
Treating the cells with DTT
A positive DAT
A positive indirect antiglobulin test
A patient’s cells and serum typed as ABO group O. The antibody screen and all panels were positive with all cells showing hemolysis at 37 degrees and agglutination after the addition of AHG. All crossmatched units reacted similarly. The same reactions were obtained at 4 degrees C and room temperature. The only negative result obtained was with the auto control and the direct antiglobulin test. What would you do next?
Test the cells with anti-H
Absorb the serum, it’s an autoantibody
Test the patient’s cell for poly-agglutin ability
Request a new sample
Test the patient’s cell for poly-agglutin ability
A mother, child, and an alleged father were HLA typed as follows:
Mother: A2. 28, B7, 35
Child: A1, 2; B8, 35
Alleged father: A1, 28; B7, 8
Choose from the following:
The man appears to be the father
The man is not the father
The test results are inconclusive
The man appears to be the father
An AB male mates with an AB female. What could be the genotype of the offspring?
BB
AA
All of the above
AB
All of the above
A freshly drawn serum sample, when tested against screen cells and panel cells hemolyzed every cell after the 37 C incubation. There were no cells left to react after washing and adding AHG. Which of the following antibodies could cause this type of reaction?
All of the above
Anti-Le(a)
Anti-A,B
Anti-Vel
All of the above
Secretor studies were performed on a patient to determine his ABO type. The tech found A blood group substance and H blood group substance. All of the controls worked. What is the patient’s ABO blood type?
Group B
Group O
Group A
Group AB
Group A
A sample of red blood cells from a healthy blood donor was typed with Lewis antisera with the following results:
Anti-Le(a): O
Anti-Le(b): 2+
What substance(s) is (Are) in the secretions?
Le(A) and Le(b) blood group substance
Neither of the Lewis blood group substances
Le(b) blood group substance only
Le(a) blood group substance only
Le(A) and Le(b) blood group substance
A patient’s history showed the following antibodies: Anti-D, Anti-Fy(a), Anti-M and anti-Jk(a). Which might not be detected in the sample because it is notorious for becoming non-detectable over time?
Anti- Fy(a)
Anti-Jk(a)
Anti-D
Anti-M
Anti-M or Jk(a)
What is an advantage of using monoclonal anti-D?
Provides a low-protein medium and decreases chances of spontaneous agglutination
Rh control is never required
Eliminates reactions caused by rouleaux in unwashed samples
Du testing eliminated
Provides a low-protein medium and decreases chances of spontaneous agglutination
Persons who have inherit the In(Lu) gene show a decreased expression of which of the following antigens:
Fy(a)
Le(a)
Jk(a)
P1
P1
If anti-N is identified in the serum of a group B person, what might their reverse (serum) ABO grouping be incorrectly interpreted as?
O
AB
A
B
O
The Jk(a) and Jk(B) antigens exhibit dosage. Therefore, if a person inherits the homozygous genotype Jk(a+b-), their red cells will react ________ with anti-Jk(a) than a person with a heterozygous genotype of Jk(+B+).
Mixed field
None of the above
Weaker
Stronger
Stronger
A patient’s cells were reported as follows: Rh: 1, -2, 3, 4, 5. What antibody can he make?
Anti-e
Anti-C
Anti-D
Anti-E
Anti-C
A patient’s cell suspension was suspended in their own plasma instead of using saline, and then used to perform the ABO type. The patient typed as group O. This same cell sample was also used for testing with anti-H, and the results were negative. All controls reacted appropriately. The antibody screen was negative at room temperature, 37C, and AHG. Choose from the following.
The patient’s cells should have been washed prior to testing
The anti-H was deteriorated
The sample may be contaminated
The patient has Bombay phenotype
The patient has Bombay phenotype
If an Rh (D) positive male mates with an Rh (D) positive female, what percent of their offspring could be Rh negative- statistically speaking?
50%
75%
25%
None
None
A sample of blood was found to have anti-Jk3. That means the person’s red blood cells were negative for both Jk(a) and Jk(b). In which of the following ethnic groups would you have the best chance of finding red blood cells that are Jk(a-b-)?
Asian
American Indian
English
African
Asian
Most Rh antibodies are of what immunoglobulin class?
IgM
IgG
IgA
IgE
IgG
All of the following may cause false negative reaction in Rh typing EXCEPT:
Failure to follow manufacturer’s directions
Resuspension too vigorous
Reagent deterioration
Rouleaux
Rouleaux
A group A woman and a group B man had a child together. What percent of the offspring could be group O?
25%
100%
50%
75%
25%
The anti-D found in chemically modified antisera reacts with D positive red blood cells at immediate spin. Explain.
The IgG antibody is mixed with high molecular weight substances so that it will react at immediate spin
The anti-D is an IgM antibody which reacts best at immediate spin
The antibody is actually IgG antibody in which the hinge region has been broken so that it can span the repulsive distance between red cells
The reagent is a mixture of monoclonal IgM and polyclonal IgG antibodies; Therefore, it will react at immediate spin
The antibody is actually IgG antibody in which the hinge region has been broken so that it can span the repulsive distance between red cells