Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

IMMUNE1 LAB MIDTERM AND FINALS > EXPERIMENT 13, 14, 15 > Flashcards

EXPERIMENT 13, 14, 15 Flashcards

1
Q

is useful in the diagnosis of Streptococcal infection and its
relative effects on organs, especially the heart and kidneys.

A

Anti – Streptolysin O (ASO) test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

is a corkscrew – shaped, gram – negative rod that infects the mucous layer of the stomach.

A

Helicobacter pylori

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Helicobacter pylori is the most common of (?), and is etiologically involved in gastric ulcer, duodenal ulcer, gastric adenocarcinoma and primary gastric B – cell lymphoma.

A

gastritis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Hepatitis B virus is a member of the (?). It is a (?) virion, with an (?) nucleocapsid core containing a rapidly (?) DNA genome.

A

Hepadnaviridae family

42 mm enveloped

icosahedral

double stranded circular

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

The envelope contains a protein called the (?), which is important for laboratory diagnosis and immunization.

A

surface antigen (HBsAg)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

is the earliest serological marker that indicates the presence of acute infection.

A

HBsAg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

HBsAg is also indicative of

A

chronic infection.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

13

REAGENTS:

A

ASO latex reagent
Positive control
Negative control
Normal Saline Solution (0.85 – 0.90% NaCl)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

13

SAMPLE:

A

Serum (should be free from contamination, hemolysis and lipemia)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

13

PRINCIPLE:

A

Latex Agglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

14

REAGENTS:

A

Test kit (cassette) for Helicobacter pylori antibody test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

14

SAMPLE:

A

Plasma or serum (should be free from contamination, hemolysis or lipemia)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

14

PRINCIPLE:

A

Immunochromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

15

REAGENT:

A

Test kit (cassette) for HBsAg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

15

SAMPLE:

A

Plasma or serum (should be free from contamination, hemolysis and lipemia)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

PRINCIPLE:

A

Immunochromatography (Solid Phase “Sandwich” Immunoassay)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

The (?) is a rapid slide agglutination test for the direct detection and semi – quantitation of anti – streptolysin O.

A

anti – streptolysin O (ASO) latex test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

the antigen, a particulate latex suspension coated with streptolysin O, agglutinates in the presence of specific antibodies present in the sera of patients with

A

Streptococcal β – hemolytic infection.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Helicobacter pylori antibody test (?) in a cassette format.

A

chromatographic lateral flow test device

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Colloidal gold conjugated Helicobacter pylori antigens are dry – immobilized at the end of nitrocellulose membrane strip.

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Helicobacter pylori antigens are bound at the test zone (T).

A
22
Q

when the sample is added, it migrates by capillary diffusion rehydrating the gold conjugate.

A
23
Q

If anti – Helicobacter pylori antibodies are present in the sample, the antibodies will bind to the gold conjugated antigens forming particles.

A
24
Q

These particles will continue to migrate along the strip until the test zone (T) where they are captured by Helicobacter pylori antigens generating a visible red line.

A
25
Q

If there are no anti – Helicobacter pylori antibodies in the sample, no red line is formed in the test zone (T).

A
26
Q

A built – in control line will always appear in the control zone (C) when the test has been performed properly, regardless of the presence or absence of anti – Helicobacter pylori antibodies in the specimen.

A
27
Q

HBsAg test is a colloidal gold enhancement immunoassay that detects hepatitis B surface antigen in human serum or plasma.

A
28
Q

The sample initially reacts with the monoclonal antibody – colloidal gold conjugate on the sample pad.

A
29
Q

This mixture migrates across the membrane by capillary action and reacts with the anti – HbsAg in the test region.

A
30
Q

If the sample contains HBsAg, a line will form on the membrane at this point.

A
31
Q

If the antigen is not present in the sample, no line is formed, indicating a negative result.

A
32
Q

The mixture continues to flow to the current area of the membrane, where it forms a line indicating the test result is valid.

A
33
Q

13

Using a semi – automatic pipette, add (?) of normal saline solution to circles 2, 3, 4 and 5

A

50 µL

34
Q

Add (?)of patient sample to circle 1 and 2

A

50 µL

35
Q

Transfer (?) of mixture from circle 2 to the saline in circle 3.

A

50 µL

36
Q

Perform serial dilution in the same manner until the last circle, discarding (?) at the end

A

50 µL

37
Q

Using the disposable pipette or stirrer, spread the diluted sample over the entire area of each circle starting at (?) and moving backwards to the next, reaching until (?)

A

circle 5

circle 1

38
Q

Add (?) latex reagent to each circle containing the sample

A

one (1) drop

39
Q

Rotate the test cards or agglutination slides at

A

100 rpm for 2 minutes

40
Q

Guide in the Interpretation of Results:
Examine macroscopically for the presence of or absence of clumps or agglutination within (?) after removing the test card or agglutination slide from the rotator

A

1 minute

41
Q

The presence of visible agglutination indicates the

A

presence of anti – streptolysin O ≥ 200 IU/mL

42
Q

✓ Negative result
✓ Positive result:

A

✓ Negative result No agglutination
✓ Positive result: Presence of agglutination

43
Q

Computation:
ASO (IU/mL) =
Limit of sensitivity =

A

limit of sensitivity x highest dilution with positive reaction

200 IU/mL

44
Q

ASO titers < 200 IU/mL are found in (?) of the healthy adult population, with values higher (up to 300 IU/mL) among children

A

95%

45
Q

14

Dispense [?] of the sample to the center of the sample well (marked as “S”)

A

3 drops (120 – 150 µL)

46
Q

Distinct colored band appears on the Test region (T) in addition to a colored band on the Control region (C)

A

POSITIVE

47
Q

No colored band appears on the Test region (T) but distinct colored band appears on the Control region (C)

A

NEGATIVE

48
Q

Neither Test (T) nor Control (C) colored band appears, or colored band only appears on the test region (T) but not on the Control region (C)

A

INVALID

49
Q

The specimen should be tested again using a new device

A

INVALID

50
Q

15

Dispense [?] of the sample to the center of the sample well (marked as “S”)

A

3 drops (100 µL)

IMMUNE1 LAB MIDTERM AND FINALS (16 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions