Test 3 Last Minute Flashcards
Explain the cell fusion experiment and the microinjection experiment. What is the conclusion of these two results?
The cell fusion experiment involved taking two cells in M-phase and interphase and then creating a heterkaryote. They found that the interphase cell had condensed into chromosomes.
The microinjection experiment took the cytoplasm of the M-phase cell and then injected into the interphase cell of G2 phase. This cause the formation of an early meiotic spindle
Conclusion: there is some regulatory molecule in the cytoplasm of the M-phase cell which allows for mitosis to occur
Steve Jobs
-affinitior drug
-Pim 1 biomarker for pancreatic research
-pancreatic cancer immunotherapy
Explain the laser beam experiment
There is a photobleached r
Anchorage dependencen and density-dependent inhibition?
Healthy cells demonstrate ancorage dependence
-they must have some medium to start cell growth
DDI
-they form one layer and then stop in the G0 phase
Meelhson and Stahl
They grew a generation of E.coli in 15N and then took that and replicated once with 14N
Observed that:
15N only = heavy band
14N + 15N = one band, lighter
Many rounds with 14N = heavy band disappeared, the 15N + 14N and only 14N band appears in 50/50
Explain chromosome flouresence as proof for the semi-conservative model
The chromosome flouresence is proof because it shows that there are some regions with light and some with no flouresence at all
15N = dyed
There will only be intermediate and only 14N left so some regions will be dark and some mixed
Tandem repeats
A sequence of two or more bases which are repeated thousands of times in noncoding regions of the DNA
Three different fates of an SNP
-in a noncoding region
-close to a gene acts like a DNA marker
-linked to a gene
Explain all steps of meiosis I briefly
Prophase I
Metaphase I
Anaphase I
The proteins holding together the homologous chromosomes will breakdown and split and enlongate
Telophase I
