Last minute review flashcards - Bio test 2
Explain deacylation
Deacylation is the process of removing acetyl groups from neighbouring histones using the HDAC protein which can bind onto methylated DNA.
It then returns the nucleosomes back to normal and lead to masking of enhancer and promoter sequences of DNA to repress transcription.
What is the only thing that can bind to methylated DNA
Why is it important that this binds?
The only thing that can bind onto methylated DNA is the HDAC protein. This must be done to further inhibit transcription by removing acetyl groups from neighbouring histones and returning the gene back to it’s normal off state.
Explain the process of in situ studies
For in situ studies the target gene will be taken and its mRNA will be extracted. Then a flourescent probe/complementary DNA strand is going to be made and it’s going to show up in certain tissue/cell regions.
Explain the process of in vitro studies
Take out the mRNA from the gene of interest and then use a reverse transcriptase enzyme to form a complenentary cDNA molecule with flouresecent nulcoetides.
Then you are going to place this cDNA in a microarray tray with single-stranded DNA fragments of each gene of interest
Allow them to hybridize and see how they interact with genes
Default state of transcription in prokaryotes and eukaryotes
prokaryotes = on
eukaryotes = off
What is it the time a protein has in a cell called?
Selective degredation
Explain what the regulatory promoter is going to do
Its going to bind transcriptional activator proteins onto enhancer regions and then become bound to the basal mahinery @ core promoter region through a mediator complex forming the transcription initation complex.
Regulatory sequence in prokaryotes
Promoter + operator region
-controls transcription of genes
Two enzymes coded by the lac operon
-B-galactosidase enzyme
-Lactose permease
The amount of cAMP in a cell will determine two factors:
- the degree of positive regulation (like if lactose is present and induces the repressor protein so it can’t bind the cAMP cells will just increase that degree and make the expression of genes even higher) and it allows for RNA polymerase to bind better
- whether it will bind to the CRP protein or not
What is the gene in humans for lactose metabolism?
What about the enhancer gene for this?
Lactase enzyme
Made by the LCT gene -> produces lactase on chromosome 2
MCM6 is the enhancer gene
If someone is lactose intolerant how can you explain this on a genetic level?
Lactose intolerant
Galactosemia
Have lactase enzyme
Can’t break down 1/3 enzymes GALE, GALT, GALKI due to mutation in one of these genes
Double negative feedback
A lactose molecule binds onto a repressor protein acting as an inducer molecule/allosteric inhibitor.
This is double negative feedback becasue it is inhibiting the negative feedback
What did Ellie Metchnikoff propose?
That bacteria has a positive role on the body
Populations that lived on fermented milk lived longer because it would “seed” intestine with lactic acid producing bacteria which lowers pH and kills bad bacteria