1.15 Expressing mutated genes to study rare diseases Flashcards
heterologous expression
The targeted introduction and subsequent functional translation of a gene from one organism into the host cell machinery of another, often enabling protein production for research or therapeutic purposes.
polymerase chain reaction (PCR)
Repeatedly copying a specific DNA segment using “molecular photocopying” to generate millions of identical copies for detailed study.
agarose gel
A wobbly racetrack for DNA molecules, separating them by size for easy identification.
expression vector
A DNA “taxi” carrying your gene and its “instruction manual” to the cellular machinery to get them working in a new cell type.
inducible pluripotent stem cells (iPSCs)
Reprogrammed cells that rewind to youthful potential, offering a versatile lab toolkit for studying health and disease.
