Lecture 5 Questions

Question 1

By which means can nucleosomes contribute to gene regulation / other DNA associated processes?

Answer 1

Histone modifications
DNA methylation

Question 2

What are the functions of nucleosomes?

Answer 2

DNA packaging and access to DNA
Modulating Transcription
DNA stability

Question 3

What are direct and indirect mechanisms by which histone PTMs function?

Answer 3

Direct: Histone acetylation/methylation
Indirect: PTM crosstalk / modifying Chromatin remodelers

Question 4

What kind of information can be ‘encoded’ by histone PTMs (e.g. functional elements)?

Answer 4

Gene Activity (acetylation/methylation of histones)
Promoter and Enhancer Elements
Epigenetic Inheritance

Question 5

Which effects would be expected if histone chaperones are lost?

Answer 5

Defective DNA replication / repair / transcription / nucleosome assembly

Question 5

Example roles of histone chaperones and histone variants?

Answer 5

Nucleosome Assembly and Disassembly
Histone Exchange and Turnover

Modulation of Chromatin Structure (H2A.Z)
Regulation of Transcription (H3.3)
Disease and Pathogenesis