Autotrophic Nutrition Flashcards

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1
Q

What is Autotrophic nutrition?

A

This is the process by which organisms take in inorganic carbon and energy, to form complex organic compounds.

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2
Q

Explain the types of Autotrophs

A

(1) Phototrophs - organisms which synthesize organic compounds using light energy. e.g. all green plants, algae, cyanobacteria, blue-green bacteria, green sulphur bacteria, purple sulphur bacteria, colourless sulphur bacteria.

(2) Chemotrophs - organisms which synthesize organic compounds using energy extracted from oxidation of inorganic chemicals by the process called chemosynthesis e.g. Nitrosomonas and Nitrobacter

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3
Q

What is Chemosynthesis?

A

This is a chemical process in which inorganic chemicals are oxidized to provide energy to living organisms for the synthesis of organic compounds.

The chemosynthetic bacteria utilize the energy from the chemical oxidation of inorganic chemicals to synthesize organic compounds, some of which are subsequently oxidized in respiration to yield energy for metabolism.

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4
Q

Define photosynthesis

A

It is the formation of complex organic substances inside the cell containing chlorophyll from carbon dioxide and water using sunlight energy.

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5
Q

What is the importance of photosynthesis?

A
  1. It is the means by which the sun’s energy is captured by plants for use by all organisms.
  2. It provides a source of complex organic molecules for heterotrophic organisms.
  3. It releases oxygen for use by aerobic organisms.
  4. It reduces on gaseous carbon dioxide, which would accumulate in the atmosphere to cause green house effect.
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6
Q

What are the adaptations of leaves to obtaining sunlight?

A
  1. Phototropism causes shoots to grow towards light in order to obtain energy.
    2.Etiolation causes rapid elongation of shaded shoots to enable access to light.
  2. The mosaic leaf arrangement minimizes leaf overlap and reduces leaves shading each other.
  3. Leaf large surface area enables capturing maximum sunlight.
  4. Thinness of leaves enables maximum light penetration.
  5. The transparence of leaf cuticle and epidermis allow light penetration into the photosynthetic mesophyll.
  6. The palisade mesophyll cells are densely packed with chloroplasts to trap much light.
  7. Cyclosis (movement of chloroplasts within the mesophyll cells) allows repositioning in the direction of light.
  8. The chloroplasts hold chlorophyll in an ordered way on the sides of the grana to present maximum chlorophyll to the light and also bring it close to other pigments / substances necessary for functioning.
  9. Multiple cell layers in the palisade mesophyll of sun plants increases photosynthetic efficiency.
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7
Q

What are the adaptations of leaves for gas entry and exit?

A
  1. Numerous stomata are present in the epidermis of leaves to enable entry and exit of gases.
  2. The guard cells bordering stomata pores can be opened and closed to regulate the uptake of carbon dioxide and the loss of water.
  3. Spongy mesophyll possesses many airspaces to enable faster and uninterrupted diffusion of gases between the atmosphere and the palisade mesophyll which wouldn’t happen if the gases were to diffuse through the cells themselves, a process which would be much slower.
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8
Q

What are the adaptations of leaves for liquid entry and exit?

A
  1. A large central midrib containing a large vascular bundle comprising xylem and phloem tissue is possessed by most dicotyledonous leaves for the entry and transport of water and mineral salts, and the phloem for carrying away sugar solution, usually in the form of sucrose.
  2. A network of small veins is found throughout the leaf to ensure that every cell is close to xylem vessel or phloem sieve tube for constant supply of water for photosynthesis and a means of removing the sugars they produce.
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9
Q

What are the adaptations of chloroplasts to their functions?

A
  • Outer membrane is semi- permeable to regulate entry and exit of substances for maintaining internal chloroplast environment.
  • Abundant light trapping pigments for photosynthesis
  • Abundant enzymes catalyse photosynthetic reactions in the stroma.
  • Extensive network of thylakoid membranes increase surface area for photosynthesis.
  • Narrow intermembrane space enables H+ ion concentration gradient to be rapidly established for chemiosmosis to occur
  • Inner membrane contains molecules for electron transport pathway
  • DNA presence codes for protein synthesis, including enzymes.
  • Many ribosomes for protein synthesis to reduce on importing proteins from cytoplasm.
  • Outer membrane is permeable to gases like carbon dioxide which is a raw material for photosynthesis.
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10
Q

What are the two types of photosynthetic pigments?

A
  • Chlorophyll (alpha and beta)
  • Cartenoids (accessory pigments; hand over energy absorbed to chlorophyll a)

Function of both is to absorb light
- Cartenoids also protect chlorophyll from photo damage

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11
Q

What is the difference between incandescence and luminescence?

A

Incandescence is the emission of light from hot matter e.g. the sun. (The hotter the material, the shorter the wavelengths of emitted light, the more the energy)

While
Luminescence is the emission of light when ‘excited’ electrons fall to a lower energy, emitting a photon e.g. the light- emitting diode bulbs in school labs, fluorescent lights, light from leaf extracts, etc.

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12
Q

What are the three ways in which light interacts with a leaf?

A
  1. Reflection (Reflectance): light can simply rebound off the leaf surface and hence never utilized in leaf photosynthesis.
  2. Transmission (Transmittance) through the leaf, exiting from the underside.
  • Transmission depends on the thickness of the leaf; thin leaves transmit more light than thick leaves
  1. Absorbance by the leaf, in which case the light might be used in photosynthesis.
    - Of the absorbed visible radiation, 70%, is used in photosynthesis while 30% is transmitted through the leaf.
    - Blue and red are the most absorbed wavelengths, and green and far infrared wavelengths pass through.
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13
Q

True or false
The amount of sunlight decreases as light penetrates down the vegetation layers

A

True
This is because the amount of leaf area increases.

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14
Q

What does magnesium deficiency in plants cause?

A

Chlorosis

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15
Q

What issues are faced by plants in different light intensities?

A
  • In low light, plants need to maximise light absorption for photosynthesis to exceed respiration if they are to survive.
  • In high light environment, plants maximise their capacity for utilising abundant light energy, while at the same time dealing with excess sunlight which can bleach chlorophyll.
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16
Q

What are the adaptations of shade plants to photosynthesis in their habitat?

A
  1. Abundant chlorophyll b (low chlorophyll a to chlorophyll b ratio) which gives leaves dark green colour to increase light absorption in the dark;
  2. Palisade/ spongy mesophyll ratio low (few palisade, more spongy) to allow maximum light penetration;
  3. Mesophyll cell surface / leaf area ratio low to maximise light trapping;
  4. Leaf orientation horizontal to maximise light trapping;
  5. Reddish leaf undersides to enhance reflectance back up through the photosynthetic tissue; giving the plant a second chance to utilize the light.
  6. Stomatal density low to avoid over cooling;
  7. Thin leaves to maximise light penetration;
  8. Stomatal size large to allow loss of excess water;
  9. Elongated internodes for increased access to light;
  10. Chloroplast size large to increase the surface area for storage of photosynthetic pigments.
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17
Q

What are the adaptations of sun plants to photosynthesis in their habitat?

A
  1. Abundant chlorophyll a (high chlorophyll a to chlorophyll b ratio) to increase light absorption;
  2. Palisade/ spongy mesophyll ratio high to minimise light penetration;
  3. Mesophyll cell surface / leaf area ratio high to minimise excessive light and transpiration;
  4. Leaf orientation erect to minimise light trapping;
  5. Stomatal density high to avoid over heating;
  6. Much carotenoids to prevent damage to chlorophyll from very bright light.
  7. Thick leaves to minimise light penetration
  8. Stomatal size small to minimise water loss;
  9. Much chlorophyll
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18
Q

Why is the ratio of chlorophyll a : chlorophyll b is bigger in sun leaves than shade leaves?

A

Sun leaves contain more chlorophyll a than shade leaves because chlorophyll a is more effective at absorbing the light wavelengths available to sun leaves e.g. about 450 nm.

Shade leaves contain more chlorophyll b than sun leaves because in shade plants chlorophyll b improves light- capturing capability of the chloroplast.

Sun leaves contain more carotenoids than shade leaves because carotenoids are accessory pigments that shield chlorophylls from destruction by excessive sunlight.

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19
Q

Why is it that few species of plants can survive under shady habitats?

A
  • Less direct light reaches ground via gaps in the canopy hence minimum energy is available for effective photosynthesis.
  • Of the light that passes through leaves, only a small range of wavelengths reaches the ground, which is not effective for photosynthesis.
  • Therefore, under shady habitats little light energy is available for chlorophyll to absorb and hence photosynthesise is insufficient for growth.
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20
Q

Describe the chlorophyll molecule structure

A
  • Chlorophyll molecule has a tadpole-like structure
  • A hydrophilic head
  • A hydrophobic tail made up of long chain alcohol.
  • The flattened head is made up of four nitrogen rings
  • The skeleton of each pyrrole ring is made up of 5 atoms - four carbon and one nitrogen.
  • The nitrogen lies towards the centre.
  • A magnesium atom is held in the centre of porphyrin head
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21
Q

What is the absorption spectrum of photosynthetic pigments?

A

It is a graph of the relative absorption of different wavelength of light by a pigments like chlorophyll.

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22
Q

What is the action spectrum of photosynthesis?

A

A graph of the effectiveness of different wavelengths of light in stimulating the photosynthetic process.

It represents the actual rate of photosynthesis in living cells.

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23
Q

What are the two stages of photosynthesis?

A
  • Light dependent stage
  • Light independent stage (Calvin’s cycle)
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24
Q

Where does the light independent stage of photosynthesis take place?

A

Thylakoids in the chloroplasts

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25
Q

What are the products formed after the light independent stage of photosynthesis?

A
  • ATP
  • NADPH
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26
Q

What is the main function of the light independent phase of photosynthesis?

A
  1. Photophosphorylation i.e. addition of an inorganic phosphate to Adenosine diphosphate (ADP) to form Adenosine triphosphate (ATP) using light energy.
  2. Formation of NADPH+ which is the reduced form of Nicotinamide adenine dinucleotide phosphate.
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27
Q

Describe the light sensitive stage of photosynthesis

A
  • Light strikes photosystem 2 and 1 and the chlorophyll molecules within those systems absorb the light energy
  • Chlorophyll molecules release high electrons and these are excited and boosted to higher energy levels
  • The excited electrons are captured by and electron acceptor
  • The electron acceptor is reduced and the chlorophyll molecule is left positively charged (oxidized)
  • The electron travels from one electron acceptor to another in a series of oxidation-reduction reactions.
  • Energy lost during this electron flow facilitates formation of ATP
  • In cyclic photophosphorylation, electrons may pass back to the chlorophyll molecule by the electron carrier system, yielding ATP as they do so
  • In non-cyclic photophosphorylation, The electrons which have been lost by the chlorophyll molecule are regained by splitting of a water molecule into electrons, protons and oxygen gas
  • Oxygen gas is hence the byproduct of photosynthesis
  • The protons from the water then combine with electrons from the second electron acceptor and these reduce NADP to NADPH used in the light independent phase
28
Q

Define chemiosmosis

A

It is the movement of ions across a selectively permeable membrane down an electrochemical gradient.

29
Q

What are the similarities between cyclic and non-cyclic photophosphorylation?

A

In both:
(1) there is flow of electrons through several electron carriers

(2) there are pigment systems which accept and lose electrons

(3) ATP is formed

(4) pigment system I is involved

(5) electron movement is located in the thylakoid membranes

(6) protons are moved outwards of the thylakoids

(7) protons (H+) are actively pumped from stroma into thylakoid space

(8) there is photo-excitation of electrons in the pigment systems.

30
Q

What are the differences between cyclic and non-cyclic photophosphorylation?

A

Non-cyclic
- Electrons flow unidirectionally (non-cyclically)
- First electron donor is (source of electrons) water
- Last electron acceptor is NADP
- The products are ATP, NADPH and Oxygen
- Involves both pigment systems I and II
- Photolysis of water occurs

Cyclic photophosphorylation
- Electrons flow cyclically
- First electron donor is pigment system I (PSI)
- Last electron acceptor is pigment system I (PSI)
- The product is ATP only
- Involves only pigment system I
- No photolysis of water

31
Q

What is the role of water in photosynthesis?

A
  • Catalytic photolysis / splitting / breaking of water produces electrons (e-) and protons (H+).
  • Water is a source of electrons to replace those lost by chlorophyll / photosystem II
  • Water is a source of H+ needed to produce NADPH + H
  • Water is a source of H+ which when flowing from thylakoid space into stroma via ATPase, ATP forms.
  • Water is a substrate / reactant / raw material / for photosynthesis
  • Water is transparent so photosynthesis can take place underwater / light can penetrate to chloroplasts
32
Q

Why is the Calvin’s cycle also called the dark reaction?

A

This is because does not require light, although can take place in light also.

33
Q

Where does the light independent stage of photosynthesis occur?

A

In the stroma of chloroplasts

34
Q

What are C3 plants?

A

These are plants whose first stable product of photosynthesis is a 3-carbon organic compound called glycerate-3- phosphate (triose phosphate)

35
Q

What is the end product of the Calvin cycle/ photosynthesis?

A

Glycerate-3-phosphate or Triose phosphate (TP)

36
Q

Describe the process of the light dependent phase of photosynthesis

A
  • Carbon dioxide diffuses into the leaf through the stomata and dissolve in the moisture on the walls of the palisade cells
  • It diffuses into the cell via its membrane and further into the stroma of the chloroplast
  • Carbon dioxide combines with a 5-C compound called RuBP (ribulose bisphosphate) catalyzed by RuBP carboxylase enzyme to form an unstable 6-C compound
  • This compound breaks down into two molecules of a 3-C compound, 3-phosphoglyceric acid (PGA)
  • The reducing power of NADPH and energy of ATP is used to reduce PGA or GP to Triose Phosphate(TP)
  • Out of these, only 1/6 of the molecules of TP undergo isomerization to form hexose sugar, starch or sucrose
  • 5/6 is recycled to produce ribulose monophosphate which reacts with ATP to form more RuBP
37
Q

What is the first carbohydrate made by C3 plants?

A

PGA (3-phosphoglyceric acid)

38
Q

Why is RUBISCO enzyme very inefficient in photosynthesis?

A
  1. RUBISCO can add approximately 3CO2 to 3 molecules of RuBP each second, which is very slow for an enzyme. To make up for this, plants produce large quantities of RUBISCO, with it composing 50% of the protein in a chloroplast.
  2. RUBISCO is not a very specific enzyme as it sometimes combines RuBP with oxygen rather than CO2 because of a relatively non-specific active site, causing photorespiration which leads to the formation of a useless oxygenated intermediate, rather than carbon dioxide fixation.
39
Q

Why does RUBISCO sometimes combine with oxygen instead of carbon dioxide?

A

It has a relatively non-specific active site, causing photorespiration

40
Q

What is the result of photorespiration in C3 plants?

A

It leads to formation of a useless oxygenated intermediate, rather than carbon dioxide fixation

41
Q

What is C4 photosynthesis?

A

This is the type of photosynthesis in which the first stable product of CO2 fixation is a four carbon compound, oxaloacetate (OAA)

42
Q

What is a C4 plant?

A

These are plants in which the first stable product of CO2 fixation is a four carbon compound, oxaloacetate (OAA) that is later reduced for further metabolism

43
Q

Give examples of C4 plants

A
  • maize
  • sorghum
  • Sugar cane,
44
Q

What is Kranz anatomy?

A

A condition in which bundle sheath cells and palisade cells of the mesophyll form two concentric layers (rings) around each vascular bundle of leaves.

45
Q

Define photorespiration

A

Oxygenation of RuBP by RuBP oxygenase (RUBP carboxylase) at high temperature, low carbon dioxide and high oxygen concentration to form an oxygen containing intermediate which prevents carbon fixation in C3 plants.

46
Q

Which conditions favor photorespiration?

A
  • High temperature
  • Low carbon dioxide concentration
  • High oxygen concentration
47
Q

How does photorespiration affect plants?

A
  • When C3 plants are exposed to low carbondioxide concentration (or high oxygen concentration) e.g. when stomata close to reduce water loss, RuBP carboxylase catalyses the reaction between RuBP and oxygen to form a 2-carbon compound; phosphoglycolate, which is oxidized to release carbondioxide.
  • Yet when the carbondioxide concentration is high, RUBISCO enzyme catalyses the reaction between RuBP and carbondioxide to form a 3-carbon compound; 3- phosphoglyceric acid, which undergoes several reactions to form sugar useful to the plant.
  • Photorespiration therefore reduces the potential yield of photosynthesis by 30-40%.
48
Q

Describe the C4 cycle in CO2 fixation

A
  1. In the presence of phosphenol pyruvic acid carboxylase enzyme, Phosphenol pyruvic acid is carboxylated inside the chloroplasts of mesophyll cells to form oxaloacetic acid.
  2. Oxaloacetic acid is reduced by NADPH to malic acid in the presence of malate dehydrogenase enzyme.
  3. From chloroplasts of mesophyll cells, malic acid is translocated (shunted) to chloroplasts of bundle sheath cells where it is decarboxylated by NADP to form pyruvic acid and carbon dioxide. The pyruvic acid produced returns to mesophyll cells for phosphorylation by ATP to regenerate PEP; the CO2 acceptor.
  4. Now the second carboxylation occurs in the chloroplasts of bundle sheath cells through Calvin cycle.
  5. RuBP of Calvin cycle is called final or secondary CO2 acceptor in C4 plants.
  6. Ribulose bisphosphate accepts CO2 produced in step 3 and to yield 3-phosphoglyceric acid as given in Calvin cycle.
  7. Some of the 3-phosphoglyceric acid is utilized in the formation of glucose-6-phosphate and sucrose while rest regenerates ribulose-1, 5 bisphosphate in the system.
  8. In the Calvin cycle 3 ATP molecules are required for the C4 plants, 5 ATP molecules are required for the fixation of CO2. For the formation of a glucose molecules, C4 plants require 30 ATP while C3 plants utilize only 18 ATP.
49
Q

What are the advantages of the C4 pathway?

A
  • C4 plants able photosynthesize at very low CO2 concentration (e.g. in dense tropical vegetation) because PEP carboxylase enzyme has a very high affinity for carbon dioxide.
  • Concentric arrangement of mesophyll cell produces a smaller area in relation to volume for better utilization of available water and reduce the intensity of solar radiations. - Photorespiration, which inhibits growth in C3 plants is avoided / reduced in C4 because
    (1) the CO2 fixing enzyme PEP carboxylase does not accept oxygen
    (2) RUBISCO enzyme inside the bundle sheath cells is shielded from high oxygen concentration by the ring of palisade cells.
  • The CO2 fixing enzymes in C4 plants are more active at hot temperature and high illumination, therefore photosynthesis occurs rapidly at low altitude, hot and brightly lit tropical conditions than in C3 plants.
  • The productivity of C4 almost four times greater than in C3 because:
    (1) of the increased rate of CO2 uptake caused by (i) large internal leaf surface area (ii) short CO2 diffusion distance (iii) CO2 steep diffusion gradients
    (2) the bundle sheath cells in which dark reactions occur have (i) a large photosynthetic surface area enabled by un-usually large chloroplasts (ii) lack of grana on which O2 would be produced, so no photorespiration.
    (3) the Palisade cells in which light reactions occur have large grana to increase the photosynthetic surface area.
50
Q

Which plant photosynthesis is favored by low altitude, hot and brightly lit tropical conditions

A

C4 plants

51
Q

What are the disadvantages of the C4 pathway?

A
  • The CO2 fixing enzymes in C4 plants are less active at cool, moist and low illumination conditions, therefore photosynthesis occurs slowly at high altitude with cool temperature and in low light intensity of temperate conditions.

NB: C4 plants grow better under hot, dry conditions when plants must close their stomata to conserve water – with stomata closed, CO2 levels in the interior of the leaf fall, and O2 levels rise

52
Q

In spite of the higher productivity of C4, which is almost four times greater than in C3, majority of plants perform C3 photosynthesis. Explain this statement fully.

A
  • CO2 concentration is a major factor determining the pathway of carbon dioxide fixation.
  • While C4 plants are more productive at low CO2 concentration, C3 plants form the dominant plant life because they are effective at high CO2, whose concentration is high in most environments and steadily increases due to increasing combustion of fossil fuels.
  • Also considering that C4 photosynthesis is more complex i.e. it involves many reactions both in bundle sheath cells and in mesophyll cell, and requires a specialized Kranz anatomy, most plants have simpler structures.
  • Therefore, unless water loss is a significant issue, C3 dominate since C3 photosynthesis is more effective.
53
Q

What are the similarities between C4 and C3 plants?

A

Both:
(1) contain RUBISCO enzyme
(2) depend on light for their reactions (3) show CO2 fixation
(4) have RuBP
(5) form several same organic products e.g. PG, PGA, sucrose
(6) have the calvin cycle

54
Q

What are the structural differences between C3 and C4 plants?

A

C3 plants
- Lack Kranz anatomy
All chloroplasts have identical structure

C4 plants
- Exhibit Kranz anatomy
- Chloroplasts are dimorphic (are in two forms) e.g. those of palisade cells have grana yet are lacking bundle sheath cells.

55
Q

What are the physiological differences between C3 and C4 plants?

A

C3 plants
- CO2 acceptor is a 5-Carbon RuBP
- CO2 fixation occurs once
- Photorespiration occurs
- Less photosynthetically efficient
- GP (glycerate-3-phosphate) is the first stable organic product
- Enzymes are more efficient at lower temperatures
- RUBISCO enzyme is used
- Compensation point is attained at higher CO2 concentration

C4
- CO2 acceptor is a 3-Carbon PEP
- CO2 fixation occurs twice
- No photorespiration
- More photosynthetically efficient
- OAA is the first stable organic product - Enzymes are more efficient at high temperatures
- PEP carboxylase enzyme is used
- Compensation point is attained at lower CO2 concentration

56
Q

What is CAM photosynthesis?

A

A type of photosynthesis in which CO2 is taken in at night via open stomata, fixed by phosphoenolpyruvate carboxylase (PEPC) into OAA, stored as organic acid (mainly malate) which is later decarboxylated during daytime.

  • CO2 is assimilated in the Calvin‐cycle when stomata are closed.
  • CAM is a modified form of C3
    photosynthesis
  • adopted as an adaptation to
    water deficit in terrestrial and epiphytic plants,
57
Q

What are examples of CAM plants?

A

(Bryophyllum), Vanilla,

58
Q

Examples of plants that carry out CAM photosynthesis

A
  • Cacti
  • sisal
  • Opuntia,
  • Kalanchoe
  • pineapples,
59
Q

What is the significance of CAM photosynthesis?

A

For terrestrial CAM plants, there is increased water use efficiency in which nocturnal stomatal opening greatly reduces stomatal loss of water as it would in day light to CAM photosynthesis.

60
Q

Describe the phases of CAM through the diurnal (daytime) course

A

Phase I: nocturnal CO
fixation (atmospheric + respiratory sources) mediated by PEPC and accumulation of malic acid within the vacuole.

Phase II: atmospheric CO2
fixation at dawn which marks the transition between C4 and C3 activity

Phase III: decarboxylation of malic acid and fixation of the regenerated CO2 by Rubisco.

Phase IV: a period of atmospheric CO2 fixation from the end of Phase III to dusk which latterly incorporates the shift from Rubisco to PEPC activity.

61
Q

What is the hill reaction?

A

The photoreduction of an electron acceptor by the hydrogens of water, with the evolution of oxygen.

62
Q

What factors affect photosynthesis? Explain

A

(1) carbondioxide concentration
(2) Light intensity
(3) Temperature
(4) Chlorophyll concentration
(5) Altitude
(5) oxygen light in aquatic
concentration
(6) Water and dissolved nutrients
(7) Enzyme inhibitors e.g. cyanide, dichlorophenyl dimethyl urea –
DCMU
(8) Salinity

63
Q

What are the major photosynthetic challenges underwater?

A

(i) reduced carbon dioxide diffusion rate, which is overcome by supplementary use of HCO3− (bicarbonate ions)
(ii) low light penetration with depth.
- The rate of light penetration in a body of water depends on suspended substances and the turbidity of the water.

64
Q

What does the principle of limiting factors state?

A

At any given moment the rate of a chemical process is limited by the one factor which is nearest to its minimum value and by that factor alone

65
Q

Define compensation point

A

The light intensity at which the photosynthetic intake of carbon dioxide is equal to the respiratory output of carbon dioxide.

It occurs during early morning or late evenings

66
Q

What is the relationship between photosynthesis and respiration?

A
  • In the presence of light, plants respire aerobically to release carbon dioxide while consuming oxygen, and at the same time photosynthesise to release oxygen while consuming carbon dioxide, although photosynthesis far exceeds respiration.

-In darkness, plants respire aerobically to release carbon dioxide but photosynthesis is inhibited by absence of light.