Assisted Reproduction Techniques

Question 1

Define: Assisted Reproduction

Answer 1

Any procedure above and beyond natural mating used to attain or maintain a pregnancy OR alter normal reproduction processes or function

Question 2

Define: Superovulation

Answer 2

Stimulation of development and ovulation of multiple follicles
Primarily in ruminants for embryo transfer

Question 3

How is superovulation accomplished?

Answer 3

Administration of gonadotropin hormones
FSH
eCG

Question 4

Define: Embryo Transfer

Answer 4

Procedure in which an embryo is removed from the uterus of one female (donor) and transferred into the uterus of a surrogate female (recipient)

Question 5

With embryo transfer, where does fertilization and early embryonic development take place?

Answer 5

In the donor oviduct and uterus

Question 6

Short Term Embryo Storage

Answer 6

Cooled
Decreased metabolism
Prolongs survival
24h storage

Question 7

Long Term Embryo Storage

Answer 7

Frozen
Liquid nitrogen
‘Forever’ storage

Question 8

In cattle, the embryo must be implanted on the _______ side of the corpus luteum.

Answer 8

Same

Question 9

What embryo sex is preferred in cows vs horses?

Answer 9

Dairy cows = heifers
Cutting horses = fillies

Question 10

How can embryo sex be determined?

Answer 10

Embryo biopsy via aspiration or biopsy of blastomeres

Question 11

Define: In Vitro Fertilization

Answer 11

Procedure by which an oocyte is fertilized outside the oviduct

Question 12

Define: Intracytoplasmic Sperm Injection

Answer 12

A single sperm is injected into the cytoplasm of an oocyte using a micromanipulator

Question 13

Define: Cloning