19A.6 Flashcards
what happens when two amino acid molecules react together
an acid-base reaction occurs in which it is a condensation reaction where the amino acid with a lower isoelectric point would have the OH in its COOH bond join with the NH2 of the other Amino acid forming water and an organic product called dipeptide which is the two amino acids joined by a CONH bond known as the peptide bond
what is peptide bonds
it is a bond formed by a condensation reaction between the carbonyl group of one amino acid and the amino group of another amino acid
when amino acids reacts to form dipeptide the organic product will always have
two possible structure since either one amino acid molecule could lose its OH
if you form tripeptide they will have
6 possible structure
how can you represent the different possible structure of the tripeptides
by using the three letter abbreviation in the follwing way ala-glu-csy
how are polypeptide and proteins formed
by the condensation polymerisation of many amino acids.
what is the difference between the long-chains of poly peptides and of proteins
proteins have further level to there structure
what is polypeptide
a condensation monomer formed from many amino acids
what is a proteins
polypeptides that have folded into a specific shape in order to have a specific function
how can we discover the structure of a protein
- find the amino acids that are present
- find the order that they occur in the polypeptide chains
how can we break the polypeptide chains into its individual proteins
by hydrolysis of the compound via prolonged heating with concentrated HCl and H2O
because of the extreme acidic conditions in the hydrolysis of polypeptides, the amino acids formed would
have there NH2 group protonated to form NH3^+ bonds so when the polypeptide breaks into its amino acids they will each have a protonated amino group of NH3^+
What is the chemical reaction of the hydrolysis of dipeptide
page 253
so can we use chromatography to analyze the amino acids in polypeptides
by spotting the mixture of amino acids produced by the hydrolysis of the polypeptide into a chromatography paper. using a suitable solvent, each amino acid will rise to different height but since they are colourless the chromatogram can be sprayed with a developing agent so that their position can be seen, once their position are established we can then calculate the Rf value of all the points and form there we can identify the individual amino acid.
why is ninhydrin not used anymore as a deploring agent for chromatogram of mixture of amino acids
even though it works really well but the problem is that it has a
