1B: Molecular Genetics

Question 1

(T/F) In prokaryotes, operon with 2 genes transcribed from single promoter upstream of first gene in operon

Answer 1

true

Question 2

restriction enzymes recognize what type of sequences?

Answer 2

palindrome

Question 3

imprinted genes

Answer 3

expressed in a parent-specific manner

Question 4

Is reverse transcriptase or RNA polymerase used in cDNA cloning?

Answer 4

reverse transcriptase- RNA to cDNA

Question 5

The nucleotide sequence of the mRNA is the same/complementary to coding strand

Answer 5

mRNA- same as coding strand

mRNA-complementary to non-coding (non template strand)

Question 6

independent events (A and B) and for (A + B)

Answer 6

A and B: P(A) x P(B)

A or B: P(A) + P(B) - P(A and B)

Question 7

post-transcriptional modifications

Answer 7

• splicing
• polyadenylation
• 5’ capping

Question 8

post-translational modifications

Answer 8

• glycosylation
• proteolysis
• methylation
• acetylation

Question 9

promoters

Answer 9

found upstream and bind with transcription factors

Question 10

when would a protein have a nuclear localization sequence?

Answer 10

protein that activates transcription factors

Question 11

If a protein binds to promoter region, regulation occurs at what level?

Answer 11

regulation at transcription level (synthesis of mRNA)