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MCAT: Review > 5C: Separations and Purifications > Flashcards

5C: Separations and Purifications Flashcards

1
Q

Function of ebulliator

A

introduce small air bubbles to break surface tension of heated liquid and prevent superheating

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2
Q

good example of organic solvent

A

ether (non-reactive)

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3
Q

How does a protein size get effected when run in:
native
non-reducing SDS-PAGE
reducing SDS-PAGE

A
  • native: original structure
  • non-reducing: smallest monomers
  • reducing: smallest monomers with disulfide bonds broken
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4
Q

NMR spectroscopy: further left-shielded (downfield) the peak, the more electron drawing/withdrawing?

A

electron withdrawing

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MCAT: Review (31 decks)

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