2.3.2 Specimen collection and handling Flashcards

1
Q

Specimen?

A

Respiratory secretion (Sputum, Bronchial aspirate) with Acid treatment

Blood
Urine

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2
Q

How is acid treatment prepared?

A

1:10 dilution

with 0.2 KCl-HCl

Stand for 5 mins

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3
Q

This happens when an overgrowth of the normal flora from the respiratory tract will occur

A

Without acid treatment

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4
Q

Only legionella grow on the agar

A

With acid treatment

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5
Q

How do you release Legionella pneumophilia in Blood?

A

Lysis centrifugation

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6
Q

This is done in case of delay processing (Temp)

A

Respiratory samples (-70C)
Urine (2-8C) or Frozen at -20C

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7
Q

What is done in urine?

A

Antigen detection

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