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Biochemistry > Glycolysis > Flashcards

Glycolysis Flashcards

1
Q

What are the enzymes of the three irreversible steps?

A
  1. Hexokinase
  2. PFK-1
  3. Pyruvate Kinase
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2
Q

How many ATPs are produced in the liver with complete aerobic metabolism?

A

32

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3
Q

How many ATPs are produces in the muscle with complete aerobic metabolism?

A

30

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4
Q

What is the shuttle used for glycolysis in the liver?

A

Malate-Aspartate Shuttle

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5
Q

What is the shuttle used for glycolysis in the muscle?

A

Glycerol-3-Phosphate Shuttle

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6
Q

What is the rate limiting step in glycolysis?

A

PFK-1

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7
Q

How many ATPs are produced in aneraobic glycolysis?

A

2 ATPs per glucose

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8
Q

What inhibits PFK-1?

A

ATP and Citrate

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9
Q

What stimulates PFK-1?

A

AMP and F26BP

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10
Q

Which enzyme forms a high energy bond?

A

Glyceraldehyde-3-P dehydrogenase

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11
Q

What inhibits pyruvate kinase?

A

ATP and Alanine

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12
Q

What stimulates pyruvate kinase?

A

F-1,6-BP

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13
Q

Where does glycolysis take place?

A

Cytoplasm

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14
Q

Where is PFK-1 regulated?

A

Liver

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15
Q

What enzyme catalyzes F-2,6-BP?

A

PFK2

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16
Q

What enzyme coverts pyruvate to lactate?

A

Lactate Dehydrogenase

17
Q

What is the main function of LDH?

A

To regenerate NAD+ so that glucose carbons can flow through glycolysis= NADH recycling

18
Q

What enzyme has a high affinity and low capacity for glucose to G-6-P and is uninduced by insulin?

A

Hexokinase!!!

19
Q

What enzyme is found only in the liver and has a low affinity and high capacity for glucose to G6P and is induced by insulin?

A

Glucokinase

20
Q

What enzyme converts pyruvate to OOA?

A

pyruvate carboxylase

21
Q

What are the fates of pyruvate?

A
  1. AcetylCoA–> TCA (PDHC)
  2. Lactate (LDH)
  3. OOA (PC)
  4. Alanine (ALT)
22
Q

What is ATP yield of free glucose compared to glucose from glycogen in glycolysis?

A

Free glucose= 2 ATP
G-1-P= 3ATP
Don’t lose an ATP by phosphorylating it

Biochemistry (6 decks)

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