3.16 Chromatography Flashcards
(6 cards)
1
Q
How chromatography work?
A
separation depends on the affinity for mobile or stationary phase
2
Q
Thin Layer chromatography
A
Runs fast
Plates are robust
Plate is coated in silica gel- stationary phase
Solvent is mobile phase
3
Q
How do you calculate Rf value?
A
distant moved by solvent
4
Q
Column Chromatography
A
silica packed in tube- stationary phase
solvent, mobile phase is ran through tube
allows for silica to be used multiple times
allows for collection of individual constituents
5
Q
Gas Liquid Chromatography
A
stationary is a powder packed capillary tube
mobile is an inert gas
Different components leave tube at different times- retention time
accurate detectors used
6
Q
GCMS
A
Gas Liquid Chromatography with a Mass Spec attached
