3.2 cells Flashcards
what does a light microscope have
Light microscopes use a pair of convex glass lenses that can resolve images that are 0.2um apart.
how do electron microscopes work
Electron microscopes work in a similar way to light microscopes, but instead use a beam of electrons that are focused by electromagnets inside a vacuum environment, which is needed so that particles in the air do not deflect the electrons out of the beam alignment.
how do transmission electron microscopes work
has an electron gun that focuses a beam of electrons that is focused onto the specimen by a condenser electromagnet. It passes through a thin section. Parts of the specimen absorb electrons and therefore appear dark. An image is produced on a screen and can be photographed by a micrograph
how do scanning electron microscopes work
A beam of electrons is directed onto the surface of the specimen from above. The beam is then passed back and forth across a section of specimen in a regular pattern. The electrons are scattered by the specimen and the pattern of this scattering depends on the contours of the surface. A £D image can be built by computer analysis of the pattern of scattered electrons
what are the limitations of electron microscopes
- system must be in a vacuum so living specimens cannot be observed.
- A complex staining process is required which may introduce artefacts into the image.
- Specimens have to be very thin, particularly for TEM so that the electrons can pass through.
- SEM has a lower resolving power than TEM, but both have greater resolving power than a light microscope.
what is cell fractionation
Cell fractionation is the process in which different parts and organelles of a cell a separated so that they can be studied in detail.
what is the homogenate put in with cell fractionation
The homogenate at the beginning is placed in a cold, buffered solution of the same water potential as the cells. This is to prevent the organelles from bursting under osmotic pressure, to inactivate any enzymes from breaking down organelles and so that the pH does not fluctuate.
what is the cell fractionation process
- The cells are first blended in an homogeniser forming the resultant fluid called the homogenate.
- This tube of homogenate is then placed in a centrifuge and spun at a slow speed.
- The heaviest organelles, the nuclei, are forced to the bottom of the tube where a thin sediment or pellet forms.
- The fluid at the top, called the supernatant, is removed which leaves just the sediment of the nuclei. 5. The supernatant is then transferred to another tube and spun at a slightly faster speed. This time the pellet that forms contains the next heaviest organelle, the mitochondria.
- This process continues so that each time the speed is increased the next heaviest organelle is sedimented and separated out.
what is the nucleus
- Large organelle surrounded by a nuclear envelope (double membrane)
- Contains chromosomes and 1 or more nucleolus
- Nucleoplasm
- Controls cells activities
- DNA contains code to make proteins
- Nucleus pores allow substances to move in or out
- Nucleolus makes ribosomes
what is the cell wall
Rigid structure that surrounds the plasma membrane
In plants and algae- made of mainly cellulose
Fungi- made of chitin
Supports cell and keeps it’s rigid shape
Where two plant cell walls come together, it forms a middle lamella and strands of cytoplasm called plasmodesmata reach across to that cell
The plasmodesmata communicates between cells.
what is mitochondria
Double membrane
Inner membrane folds to form cristae
Matrix inside contains enzymes involved in respiration
Site of aerobic respiration – produces ATP (energy source)
Large number in active cells
what is rough endoplasmic reticulum
System of membranes
Surface is covered in membranes
folds and processes proteins made at the ribosomes
what is smooth endoplasmic reticulum
similar to RER but has no ribosomes
Synthesizes and processes lipids
Transport system
what is lysosomes
Type of Golgi vesicles with no clear internal structure Contains lysozymes( digestive enzymes) can be used to digest invading cells and break down old cells components
what is golgi apparatus
Group of fluid-filled sac in cytoplasm-bound flattened sacs
Processes and packages new lipids and proteins
Makes lysosomes
what is golgi vesicles
Small fluid-filledsac in cytoplasm, surrounded by a membrane
Produced by the Golgi apparatus
Stores lipids and proteins make by the Golgi apparatus and transports it out of cells
what is chloroplast
Found in plants and algal cells
Surrounded by a double membrane
Membrane inside is called thylakoid membranes and are stacked to form grana and linked together by lamellae
Site of photosynthesis
what is cell vacuole
Contains sap-sugar and salts
Surrounded by a membrane called a tonoplast
Maintains pressure inside cells and keeps them rigid
Isolates unwanted chemicals
When full its turgid
When empty its plasmolyzed
Vacuoles are very important for osmotic activity
what is ribosomes
Very small (70S in prokaryotes and 80S in eukaryotes)
Floats free in the cytoplasm or attached to the rough endoplasmic reticulum
Made up of proteins and rRNA
Made up of two sub-units
not surrounded by a membrane
Site of protein synthesis
what is bacteria
Versatile, adaptive and successful
Success is due to small size( 0.1-10µm)
Cellular structure is relatively simple
Cell wall is made up of murein
Murein is a polymer of polysaccharides and peptides
Many protect themselves by secreting a capsule of mucilaginous slime
Ribosomes are smaller- 70S
Store food reserves as glycogen granules and oil droplets
Genetic material is in the form of circular strand of DNA. Sperate from this, are smaller circular pieces of DNA called plasmids
Can reproduce independently and give bacterium resistance to harmful chemicals such as antibiotics
Plasmids are used extensively as vectors( carrier of genetic material) in genetic engineering.
what is the characteristics of viruses
A-cellular, non-living particles
Smaller than bacteria (20-300nm)
Contain nucleic acids such as DNA and RNA as genetic material but can only multiply in living cells
Nucleic acid is enclosed within a protein coat called the capsid.
Some viruses (such as the human immunodeficiency virus) are further surrounded by a lipid envelope, if it isn’t present, the capsid
Have attachment proteins which are essential to allow the virus to identify and attach to a host cell
how things move in/out of the membrane
Some are passive- requires no ATP/energy Diffusion Facilitateddiffusion ( makes it happen) Osmosis Active- needs transport Active transport A combination may be used to give: Co-transport
what are phospholipids
Form a bilayer. Are important components of cell-surface membranes for:
The hydrophilic heads of both phospholipid layers point to the outside of the cell-surface membrane attached by water on both sides
The hydrophilic tails of both phospholipid layers point to the centre of the cell membrane, repelled by the water on both sides
Lipid-soluble material moves through the membrane via the phospholipid portion. Phospholipids functions are to
Allow lipid-soluble substances to enter and leave the cells
Prevent water-soluble substances entering and leaving the cell
Make the membrane flexible and self-sealing
proteins in transport
Are interspersed throughout the cell surface membrane
Embedded in the phospholipid bilayer in 2 main ways:
Some proteins occur in the surface of the bilayer and never extends completely across it. Act either as mechanical supports to the membrane or, in conjunction with glycolipids, as cell receptors
Others completely span the phospholipid bilayer from one side to the other. Some are protein channels, which form water-filled tubes to allow water-soluble ions to diffuse across the membrane.