# 3.3: Microscopic measurements and calculations Flashcards

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1
Q

When using a light microscope, we can measure the size of objects using what?

A

When using a light microscope, we can measure the size of objects using an eyepiece graticule

2
Q

When using a light microscope, we can measure the size of objects using an eyepiece graticule.
What is an eyepiece graticule?

A

An eyepiece graticule is a glass disc that is placed in the eyepiece of a microscope

3
Q

When using a light microscope, we can measure the size of objects using an eyepiece graticule.
An eyepiece graticule is a glass disc that is placed in the eyepiece of a microscope.
What is etched on to the glass disc?

A

A scale is etched on to the glass disc

4
Q

When using a light microscope, we can measure the size of objects using an eyepiece graticule.
An eyepiece graticule is a glass disc that is placed in the eyepiece of a microscope.
A scale is etched on to the glass disc.
This scale is typically how long?

A

This scale is typically 10 mm long

5
Q

When using a light microscope, we can measure the size of objects using an eyepiece graticule.
An eyepiece graticule is a glass disc that is placed in the eyepiece of a microscope.
A scale is etched on to the glass disc.
This scale is typically 10 mm long and is divided into what?

A

This scale is typically 10 mm long and is divided into 100 sub-divisions

6
Q

When using a light microscope, we can measure the size of objects using an eyepiece graticule.
An eyepiece graticule is a glass disc that is placed in the eyepiece of a microscope.
A scale is etched on to the glass disc.
This scale is typically 10 mm long and is divided into 100 sub-divisions.
The scale is visible when doing what?

A

The scale is visible when looking down the eyepiece of the microscope

7
Q

The scale on the eyepiece graticule cannot be used directly to do what?

A

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens

8
Q

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will do what?

A

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will magnify to a different degree

9
Q

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will magnify to a different degree.
The eyepiece graticule must first be what for a particular objective lens?

A

The eyepiece graticule must first be calibrated for a particular objective lens

10
Q

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will magnify to a different degree.
The eyepiece graticule must first be calibrated for a particular objective lens.
Once calibrated in this way, the eyepiece graticule can remain how?

A

Once calibrated in this way, the eyepiece graticule can remain in position for future use

11
Q

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will magnify to a different degree.
The eyepiece graticule must first be calibrated for a particular objective lens.
Once calibrated in this way, the eyepiece graticule can remain in position for future use, provided that what?

A

Once calibrated in this way, the eyepiece graticule can remain in position for future use, provided that the same objective lens is used

12
Q

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will magnify to a different degree.
The eyepiece graticule must first be calibrated for a particular objective lens.
Once calibrated in this way, the eyepiece graticule can remain in position for future use, provided that the same objective lens is used.
It is therefore sensible to do what?

A

It is therefore sensible to:

1. Record the results of the calibration for a particular objective lens
2. Leave this attached to the microscope
13
Q

The scale on the eyepiece graticule cannot be used directly to measure the size of objects under a microscope’s objective lens, because each objective lens will magnify to a different degree.
The eyepiece graticule must first be calibrated for a particular objective lens.
Once calibrated in this way, the eyepiece graticule can remain in position for future use, provided that the same objective lens is used.
It is therefore sensible to record the results of the calibration for a particular objective lens and leave this attached to the microscope.
This will save you having to do what?

A

This will save you having to recalibrate each time you want to measure the size of the object being viewed under the microscope

14
Q

To calibrate an eyepiece graticule, you need to use what?

A

To calibrate an eyepiece graticule, you need to use a special microscope slide called a stage micrometer

15
Q

To calibrate an eyepiece graticule, you need to use a special microscope slide called a stage micrometer.
This special microscope slide also has what etched onto it?

A

This special microscope slide also has a scale etched onto it

16
Q

To calibrate an eyepiece graticule, you need to use a special microscope slide called a stage micrometer.
This special microscope slide also has a scale etched onto it.
Usually the scale is what?

A

Usually the scale is 2 mm long

17
Q

To calibrate an eyepiece graticule, you need to use a special microscope slide called a stage micrometer.
This special microscope slide also has a scale etched onto it.
Usually the scale is 2 mm long and its smallest sub-divisions are how long?

A

Usually the scale is 2 mm long and its smallest sub-divisions are 0.01 mm, 10 μm

18
Q

When the eyepiece graticule scale and the stage micrometer scales are lined-up, it is possible to calculate what?

A

When the eyepiece graticule scale and the stage micrometer scales are lined-up, it is possible to calculate the length of the divisions on the eyepiece graticule