Chapter 20 and 21

Question 1

A DNA molecule made into vitro with segments from different sources

Answer 1

Recombinant DNA

Question 2

Manipulation of organisms or their components to produce useful products

Answer 2

Biotechnology

Question 3

Direct manipulation of genes for practical purposes

Answer 3

Genetic engineering

Question 4

Small, circular double-stranded DNA molecule that carries accessory genes separate from those of a bacterial chromosome

Answer 4

Plasmid

Question 5

The production of multiple copies of a single gene

Answer 5

Gene cloning

Question 6

DNA molecule that can carry foreign DNA into a host cell and replicate there

Answer 6

Cloning vector

Question 7

Used for ampicillin resistance

Answer 7

ampR

Question 8

Used for lactose breakdown

Answer 8

lacZ

Question 9

A set of clones containing all the DNA segments from a genome, each with a plasmid, BAC, or other cloning vector

Answer 9

Genomic library

Question 10

The process of base pairing between a gene and a complementary sequence on another nucleic acid molecule

Answer 10

Nucleic acid hybridization

Question 11

Recognize and cut a specific DNA sequence

Answer 11

Restriction enzyme

Question 12

Specific sequence of nucleotides at which a restriction enzyme cleaves a DNA molecule

Answer 12

Recognition sequence