3.6 Chemical Examination of Urine (Blood)

Question 1

Clinical significance of blood in the urine:

S______ pattern on reagent strip test; dark b___-green spots against y______-green background

Answer 1

Speckled pattern

Blue

Yellow

Question 2

Uniform/homogenous on reagent strip test; appears blue-green

T or F

Answer 2

T

Question 3

On reagent strip, there is a lysing agent for blood; the speckled pattern is caused by a concentrated number of WBCs that has lysed upon contact.

T or F

Answer 3

F

RBCs

Question 4

Differentiation of Hemoglobin and Myoglobin:

Plasma appearance:

Hemoglobinuria: Normal (Clear plasma)

Myoglobinuria: Pink-Red Plasma

T or F

Answer 4

F

Baligtad

Question 5

Differentiation of Hemoglobin and Myoglobin:

Plasma appearance:

Hemoglobinuria: Hemoglobin has a binding protein of ________________
that’s why it stay in the plasma. Free Hemoglobin will be SECRETED in URINE

Answer 5

HAPTOGLOBIN

Question 6

Differentiation of Hemoglobin and Myoglobin:

Plasma appearance:

Myoglobinuria: It does not stain the plasma since it is easily excreted

T or F

Answer 6

T

Question 7

Differentiation of Hemoglobin and Myoglobin:

Ammonium sulfate method which is a blood heim test

T or F

Answer 7

T

Question 8

In ammonium sulfate method, the test that is based in ammonium sulfate P______________

Answer 8

Precipitation

Question 9

What is the reagent for Ammonium Sulfate method?

a. 2.8g (NH4)2SO4
b. 2.8g (NH4)SO4
c. 2.8g (NH3)2SO4

Answer 9

a

Question 10

Principle of ammonium sulfate method:

Hemoglobin is precipitated by (NH4)2SO4 and is not detected in the supernatant

T or F

Answer 10

T

Question 11

Differentiation of Hemoglobin and Myoglobin:

Enzyme assays what are the markers?

C_______ K_________
L_______ D_______________

Answer 11

Creatine Kinase
Lactate Dehydrogenase

Question 12

Creatine kinase: Slightly increase
LD: increased (LD1 and LD2)

a. Hemoglobinuria
b. Myoglobinuria

Answer 12

a

Question 13

Creatine kinase: Markedly increase
LD: increased (LD4 and LD5)

a. Hemoglobinuria
b. Myoglobinuria

Answer 13

b

Question 14

In Myoglobinurua, the PRINCIPLE employed in the reagent strip testing for BLOOD is P_________ activity of HEME which is why it gives positive result in Reagent strip for blood

Answer 14

PSEUDOPEROXIDASE

Question 15

Between Hemoglobin and Myoglobin, it is ____________ that is PRECIPITATED by Ammonium Sulfate (reagent).

Answer 15

HEMOGLOBIN

Question 16

Positive for Hemoglobin: Formation of RED PRECIPITATE and Supernatant is NEGATIVE for the Reagent Strip test for BLOOD

T or F

Answer 16

T

Question 17

In cases if the present is MYOGLOBIN, it is NOT PRECIPITATED so it remains SUSPENDED. That’s why if you REPEAT the Reagent Strip Testing In Supernatant it will Turn POSITIVE

T or F

Answer 17

T

Question 18

LD1 and LD2 (Hemoglobinuria):

Isoenzymes are PREDOMINANT in _____

Answer 18

RBC

Question 19

LD4 and LD5 (Myoglobinuria)

Isoenzymes are PREDOMINANT in _______________

Answer 19

SKELETAL MUSCLES