4.5 Reagent Strip testing (Glucose) Flashcards
Principle: same as glucose oxidase-peroxidase reaction / Peroxidase-coupled glucose oxidase reaction
a. Sorensen’s Protein Error of Indicators
b. Double sequential enzyme reaction
c. Double Indicator Reaction
d. Na(Sodium) Nitroprusside reaction
e. Pseudoperoxidase activity of heme
f. Diazo reaction
g. Greiss’ Reaction
h. Ehrlich’s reaction
b
Reagents for Glucose:
a. M&C: Methyl Red & Bromthymol Blue
b. Tetrabromophenol blue
c. GOD / POD
d. Sodium nitroprusside
e. Dichloroaniline diazonium salt
f. Pdimethylaminobenzaldehyde
g. p-arsanilic acid tetrahydrobenzo-quinolinol
h. Derivative pyrrole amino acid ester
c
Chromogen for Glucose:
Potassium iodide (KI)
a. Multistix
b. Chemstrip
a
Chromogen for Glucose:
tetramethylbenzidine (TMB)
a. Multistix
b. Chemstrip
b
Color Reaction for Glucose in multistix:
a. Green, Brown
b. Yellow, Green
c. Yellow brown
a
Color Reaction for Glucose in Chemstrip:
a. Green, Brown
b. Yellow, Green
c. Yellow brown
b
Reading time for glucose?
a. 10s
b. 30s
c. 60s
b
Sensitivity/Detection Limit
75-115 mg/dL
a. Multistix
b. Chemstrip
a
Sensitivity/Detection Limit
40 mg/dL
a. Multistix
b. Chemstrip
b
Source of Interference:
Presence of strong oxidizing agents
a. F-positive
b. F-Negative
a
Source of Interference:
Ascorbic acids (Reducing agent)
a. F-positive
b. F-Negative
b
Source of Interference:
Low temp
a. F-positive
b. F-Negative
b
Source of Interference:
Old specimen
a. F-positive
b. F-Negative
b
