5.4 Microscopy Flashcards
Components:
Body tube, revolving nosepiece, arm, base
a. Body
b. Lens System
c. Illumination system
a
Components:
Light source, rheostat, condenser, iris diaphragm, field diaphragm
a. Body
b. Lens System
c. Illumination system
c
Components:
Oculars/eyepieces, objectives, coarse and fine adjustment knobs
a. Body
b. Lens System
c. Illumination system
b
What forms the so-called 1st lens system?
Objectives
What forms the so-called 2nd lens system?
Oculars/eyepiece
Manipulated to make adjustments in the intensity of light
Rheostat
Elements with low Refractive index such as hylaine casts, Mucous threads, and T. vaginalis
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
b
Routine urinalysis; use subdued light
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
a
Cholesterol-containing cells and casts; birefringent crystals
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
c
Fluorescent microorganisms
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
e
Unstained Specimens
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
d
3D microscopy image and layer by layer imaging
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
f
Detects Oval fat bodies and fatty cast
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
c
Detects Spirochetes and T. pallidum
a. Brightfield
b. Phase-contrast
c. Polarizing
d. Dark-Field
e. FLuorescence
f. Interference contrast
d
In Interference Contrast:
Differential interference contrast
a. Nomarski
b. Hoffman
a
