3.6.1 Tools of the Cytologist: Light and Fluorescent Microscopy Flashcards Preview

AP Biology > 3.6.1 Tools of the Cytologist: Light and Fluorescent Microscopy > Flashcards

Flashcards in 3.6.1 Tools of the Cytologist: Light and Fluorescent Microscopy Deck (12)
Loading flashcards...
1
Q

light microscope

A
  • also called the compound microscope, focuses and magnifies beams of light on a specimen with
    the use of lenses. The use of light provides ample magnification of an object but has limited resolution
2
Q

fluorescent microscope

A
  • a special kind of compound microscope that employs the use of fluorescent stains to increase the visibility of objects. Fluorescent microscopes are still dependent on light for illumination and are thus limited by resolution
3
Q

condenser lens

A
  • focuses a beam of light on a specimen
4
Q

ocular and objective lens

A
  • The lenses in the eyepiece and in the nosepiece, called the ocular and objective lens, respectively, bend the light and magnify the object.
5
Q

magnification

A
  • involves the extent to which the image is enlarged by the lenses
6
Q

resolution

A
  • described as the clarity of the object and is a
    measure of how close two points can be and still be
    distinguished from each other. The resolution of the pictures on the left is better than the ones on the right.
  • Resolution is dependent on the wavelength of the source of illumination. Light microscopes have limited resolution due to the fact that they employ visible light as a source of illumination.
  • Because of limited resolution, the greatest useful
    magnification that can be attained with light microscopes is 1000 times.
7
Q

fluorescent microscopy

A
  • used to improve the visibility of some structures in the cell. Fluorescent dyes or naturally fluorescing molecules in the cell are illuminated by an exciting light, such as ultraviolet light, and emit a fluorescing
    light. This light is magnified through the microscope while filtering out the exciting wavelengths. In this way the bright fluorescing structures appear on a black background and can be more easily seen in many cases than with conventional stains against a background with other colors present.
8
Q

Which of the following wavelengths of light will provide the best resolution of an image?

A
  • violet
9
Q

What is the purpose of a stain in light microscopy?

A
  • It increases the visibility of a structure.
10
Q

Which of the following is correct order of the path of light in a compound microscope.

A
  • light source, object, objective lens, ocular lens.
11
Q

The resolving power of a light microscope is limited by

A
  • the wavelengths of visible light
12
Q

When using a microscope, the property that describes the clarity of an object is called

A
  • resolution

Decks in AP Biology Class (383):