Cultivation of Viruses

Question 1

Played a vital role in virology because it provides sufficient gravitational force to efficiently sediment even the smallest viruses

Answer 1

Ultracentrifuge

Question 2

Rate Zonal Centrifugation

Answer 2

Sample is layered as a narrow zone on the top of a density gradient, under centrifugal force, particles move at different rates depending on mass

Question 3

Buoyant Density

Answer 3

Object has exactly the same density as the fluid, remains submerged in the fluid but will neither sink or float

Question 4

Isopycnic Point

Answer 4

Point where the buoyant density of a particle equals that of the surrounding density gradient medium

Question 5

Isopycnic Centrifugation

Answer 5

Particles are separated solely on the basis of their buoyant density, the particles will never sediment to the bottom of the tube, no matter how long the centrifugation time

Question 6

Membrane Chromatography