Neutrophils

Question 1

What chemokine receptors do neutrophils express?

Answer 1

CXCR1 and CXCR2

Question 2

What is the major chemokine that attracts neutrophils?

Answer 2

IL-8

Question 3

What cytokines cause dilation of blood vessels?

Answer 3

TNF-a and IL-1

Question 4

What does the endothelium express to establish weak interactions with neutrophils?

Answer 4

E and P selectin. These bind sLex on neutrophils. These are expressed on activated endothelium. Endothelium has been activated with TNFa, IL-1, or LPS.

Question 5

What type of glycoprotein do neutrophils have that interacts with selectins?

Answer 5

sLex

Question 6

What is inside out signaling?

Answer 6

Inside out signaling is caused by a chemokine (IL-8) interacting with CXCR2. This causes the integrin on the neutrophil to change conformations. This conformational change increases the strength of the integrin.

Question 7

What integrins are expressed on neutrophils?

Answer 7

LFA-1 and Mac-1

Question 8

What do LFA-1 and Mac-1 bind to?

Answer 8

VCAMs on the endothelium