Antibodies

Question 1

What portion of current therapeutics is made up by antibodies?

Answer 1

1/3

Question 2

What are the possible mechanisms of antibody action?

Answer 2

Ligand blockade, receptor blockade, receptor downregulation, depletion, signal induction.

Question 3

How can antibodies use depletion?

Answer 3

Activate MAC complex, phagocytosis, lysis and pore formation.

Question 4

Once hybridomas are produced, how can they be selected for?

Answer 4

Block the de novo synthesis of nucleotides but provide precursors for the salvage pathway. Myeloma cells cannot use the salvage pathway, but hydridomas can.

Question 5

What mechanism do antibodies use to target TNF?

Answer 5

Ligand blockade.

Question 6

What kind of antibody is Rituximab?

Answer 6

Chimeric anti-human CD20 antibody.

Question 7

How does Rituximab function?

Answer 7

Bind to CD20 and activate complement pathways, NK cells and macrophages to kill B cells.

Question 8

What kind of antibody is Adalimumab (humira)?

Answer 8

Fully human anti-TNF monoclonal antibody produced using guided selection.

Question 9

How were plants altered to produced Zmapp (ebola antibody)

Answer 9

Lack specific plant N-glycans and contain mammalian enzymes.