iPSC

Question 1

Is the K4 histone modification associate with activity of inactivity of the gene?

Answer 1

Activity

Question 2

Are the K9 and K27 histone modifications associated with active or inactive genes?

Answer 2

Inactive

Question 3

Which transcription factors transforms fibroblasts to myocytes?

Answer 3

MyoD

Question 4

List the four Yamanaka factors.

Answer 4

Sox2, Oct4, cMyc, Klf4

Question 5

List the four Jamie Thompson factors

Answer 5

Sox4, Oct9, Nanog and Lin28

Question 6

How are Yamanaka factors most commonly inserted into cells?

Answer 6

Using RNA and xenoviruses

Question 7

What is the most efficient and safest way to insert Yamanaka factors?

Answer 7

Using RNA

Question 8

What is a disadvantage of using RNA to insert Yamanaka factors?

Answer 8

RNA is unstable and needs to be reinserted every 4 days.

Question 9

How can cells be tested for pluripotency?

Answer 9

Tests for chimera formation, tetraploid complementation or teratoma formation or using in-vitro differentiation assays.

Question 10

What is the gold standard method for testing cells for pluripotency?

Answer 10

Tetraploid complementation