30. Explantation - definition, conditions, medical significance.

Question 1

Definition: to take cells (living material) from an animal or plant for placement in a culture medium

Answer 1

Definition: to take cells (living material) from an animal or plant for placement in a culture medium

Question 2

Growth factors - nutrient medium, water, vitamins, oxygen, minerals

Answer 2

Growth factors - nutrient medium, water, vitamins, oxygen, minerals

Question 3

• start to grow and divide - till they reach the bandy
• different to cancer cells: don’t stop dividing
• cells that aren’t in culture aren’t highly differentiated
• cultivation of viruses helps us make vaccinations

Answer 3

• start to grow and divide - till they reach the bandy
• different to cancer cells: don’t stop dividing
• cells that aren’t in culture aren’t highly differentiated
• cultivation of viruses helps us make vaccinations

Question 4

• examination of malignant transformations of cells: helps us to observe the behaviour of cells - malignant or benign

Answer 4

• examination of malignant transformations of cells: helps us to observe the behaviour of cells - malignant or benign

Question 5

• karyotyping - lymphocyte culture - once we extract these cells and place them in a culture medium, we can examine them in greater detail to detect chromosomes abnormalities which may cause a genetic condition

Answer 5

• karyotyping - lymphocyte culture - once we extract these cells and place them in a culture medium, we can examine them in greater detail to detect chromosomes abnormalities which may cause a genetic condition

Question 6

• transplantation immunology - mixed lymphocyte cultures. The result is DNA synthesis and proliferation of lymphocytes. Important in determining histocompatibility between donor/ recipient

Answer 6

• transplantation immunology - mixed lymphocyte cultures. The result is DNA synthesis and proliferation of lymphocytes. Important in determining histocompatibility between donor/ recipient