Flashcards in 7 - Immune System Deck (28):
What is an immune response?
The detection and destruction of antigens
What is an antigen?
A substance that causes an immune response
How do foreign cells enter the body?
-Pathogens (an organism that causes disease) - have foreign antigens and come in through food, drink, air, bodily fluids or through the skin
- An organ transplant with antigens which can be detected as foreign and destroyed by rejection - has to come from twin or close relative and person given immunosuppressants
- DNA can become damaged and the cell divides too rapidly forming a tumour and changing its antigens, the immune system therefore destroys it before it becomes cancer
- Toxins (poisons)
The destruction of foreign cells
- Phagocyte recognises foreign antigens
- Macrophage puts pseudopodia around the cell
- The Pseudopodia fuse and enclose the cell in a vacuole
- Lysosomes fuse with vacuoles, emptying hydrolytic enzymes into it
- Foreign cell digested
- Phagocyte presents the pathogens antigens
What are lymphocytes?
White blood cells with a large nucleus that almost fills the cell
- T lymphocytes (T cells) - involved in cellular response
- B lymphocytes (B cells) - involved in humeral response
- Macrophage (antigen presenting cell) engulfs pathogen
- Presents foreign antigens on its cell surface
- T Cells stimulated by specific complementary antigens on the antigen presenting cells
- Goes through clonal selection (division through mitosis)
- Can divide to form Tc cells (cytotoxic T cell) which destroys cells with foreign antigens using secreting toxic substances such as hydrogen peroxide
- Can divide to form T helper cells which release cytokines which stimulate macrophages to carry out phagocytosis and also stimulate B cells and other T cells
- B cell binds to antigen presenting cell and is stimulated
- B cells undergo clonal selection through mitosis
- Daughter cells are plasma cells and memory cells
- Plasma cells are filled with many ribosomes, endoplasmic reticulum and mitochondria so that they can produce many of the specific protein, antibodies - called monoclonal antibodies as they all come from the same cloned cell
- Antibodies stick bacteria together by agglutination which attract macrophages to destroy them by phagocytosis
Structure of antibody?
Quaternary structure, 4 polypeptides
Disulphide bridge in between two longest polypeptides
2 ends on each side are the Antigen binding sites called the variable region
Constant region below
Small chains are the light chains
Long chains are the heavy chains
Hows does immunity occur?
- Many memory cells produced by B cells
- When they come into contact with the same foreign antigen they produce plasma cells very quickly
- Plasma cells produce more antibodies faster
Primary response to infection?
When antigen enters body for first time
Slow - art many B-cells that can make the antibody and bind to it
Infected person will show symptoms
Eventually the body will produce enough antibodies
T cells and B cells produce memory cells - remain in body - Memory T cells remember the specific antigens and Memory B cells record the specific antibodies
Person is now immune
Secondary response to infection?
- Quicker, stonger
- Memory B-cells are activated and divide into plasma cells that produce the right antibody
Memory-T cells are activated that divide into the correct type of T-cells
- The foreign cell does not have enough time to divide and make us ill, we are immune
How does a vaccination work?
- Antigens in injection cause a primary response without the ability to divide and make us sick
- Creates immunity
What is active immunity?
The person is exposed to the antigen and make their own antibodies against it, creating their own memory cells
What is passive immunity?
Natural - Where someone else antibodies are put in someone to make them temporarily immune - breast milk in mothers (colostrum)
The baby does not produce its own antibodies and does not produce its own memory cells
Artificial - Injected with antibodies from someone else
What is herd immunity?
When a large % of the population is vaccinated and made immune so that a disease can't move from person to person very easily and cause an outbreak or epidemic
What does HIV stand for?
Human Immunodeficiency Virus
Structure of HIV
Retrovirus - genetic material is RNA
Contains reverse transcriptase enzyme
Phospholipid around itself called a viral envelope
Attachment proteins on outside to bind to host helper-T cell
Outside is protein called capsid
Protein capsomeres inside
Protein core inside
Replication of HIV
- Infects a particular helper T cell by using attachment proteins attaching to receptor molecule
- Capsid released into cell where it unchaste and releases the RNA
- RNA template creates a complementary strand of DNA using reverse transcriptase
- DNA codes for production of new viruses
- DNA incorporated into host cell DNA
- When the cell divides the HIV is replicated
- People who have anti-HIV antibodies in the blood are HIV positive because the antibodies can't reach the HIV in cells so they are just present in the blood
- After time, viral DNA is activates, it is transcribed and translated using the host cells own enzymes and ribosomes
- Large numbers of the virus are produced and burst out of the cells to infect new helper T cells
How does HIV harm a person?
- Number of helper T cells decreases, ability to produce antibodies decreases
- People unable to fight off infections or cancerous cells
- Said to have AIDS, Acquired Immunodeficiency virus
How to treat HIV?
- Antibiotics do not work - its a virus
- Uses antiretrovirals which bind to reverse transcriptase and stop it from copying RNA to DNA
- Keeps the quantity of the virus in the body at a low level so that the helper T cell count is maintained and the person has a working immune system
What is a monoclonal antibody?
It is a clone from a single plasma cell
How are monoclonal antibodies used in cancer treatment?
- Have complementary shapes to abnormal proteins
- Can be attached to a drug that kills cancer
- Will only deliver the drug to cancerous cells
What are two autoimmune diseases? How are they treated?
- Rheumatoid arthritis
- Crohn's disease
- TNF-a is disabled using monoclonal antibodies and the immune system attack is suppressed
How are monoclonal antibodies used in pregnancy testing kits?
- Binds to HCG which is secreted in the early months of pregnancy
- Produces colour changes on a dipstick if HCG is present
1. Monoclonal antibody that is complementary to substance is attached to enzyme
2. Antibody binds to substance
3. Dish washed to rid any unbound enzyme and antibody
4. The substrate to the enzyme is added and an enzyme-substrate complex forms to produce a colour change if the antibody did bind and the substance is present
Disadvantage of vaccination?
Can be broken down by enzymes in the gut
Why can you catch the flu multiple times?
Antigenic variability - antigens vary so the memory cells do not remember them