Molecular basis of gene transcription

Question 1

What are the structural differences between DNA and RNA?

Answer 1

DNA is double stranded, RNA is single stranded
Sugar in RNA has -OH 2’ C, DNA has -H
Thymine replaces uracil is DNA

Question 2

What direction does DNA polymerase work in?

Answer 2

5’-3’

Question 3

Describe the initiation stage of DNA transcription

Answer 3

Sigma factor binds to promoter region, Pribnow box -10 bp from start of gene (TATAAT)
Sigma factor allows RNA polymerase associate